Granulocytes antibody
| Antigen |
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| Clonality |
Monoclonal (2B2) |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN317011 |
| Quantity |
0.25 mg (1.0mg/ml) |
| Price |
410.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Granulocyte marker
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Immunogen
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Porcine bone marrow haematopoietic cells (BMHC).
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Cross-Reactivity
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Pig (Porcine)
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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2B2
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Specificity
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This antibody specifically recognises porcine basophils, neutrophils and eosinophils,acting as a reliable tool for their analysis and isolation, without contamination from othercells. Analysis of porcine bone marrow and blood shows that the antigen recognised by clone2B2 is selectively expressed on mature cells, and that it is likely to be a labileconformational epitope. Use of clone 2B2 in conjunction with clone 6D10, allows for thediscrimination and characterisation of different porcine granulocyte lineages and also theirdevelopmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immatureneutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils andbasophils. Clone 2B2 has been shown as suitable for use on cytospins.
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Application Notes
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Flow Cytometry: 1/100 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0mg/ml
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Purification
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Purified
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Buffer
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Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide (NaN3)
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
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Research Area
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Cell/Tissue Markers
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Restrictions
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For Research Use only
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Alternatives for antigen "Granulocytes", type "Antibodies"
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Hosts
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Mouse (7), Rat (4)
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Reactivities
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Human (5), Mouse (Murine) (4), Pig (Porcine) (1), Rat (Rattus) (1)
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Applications
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Immunohistochemistry (IHC) (7), Flow Cytometry (FACS) (6), Immunohistochemistry (Frozen Sections) (IHC (fro)) (6), Immunoprecipitation (IP) (5), Western Blotting (WB) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4)
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Conjugates
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FITC (2), Biotin (1)
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Epitopes
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pan (1)
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Alternatives 
