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PD-L1 antibody (CD274 Molecule) (FITC)

Details for Product anti-CD274 Antibody No. ABIN317020, Supplier: Login to see
Antigen
  • A530045L16Rik
  • B7h1
  • Pdcd1l1
  • Pdcd1lg1
  • Pdl1
  • B7-H
  • B7H1
  • PD-L1
  • PDCD1L1
  • PDCD1LG1
  • PDL1
  • RGD1566211
Reactivity
Mouse (Murine)
194
92
21
1
1
Host
Rat
96
90
58
20
2
Clonality (Clone)
Monoclonal ()
Conjugate
This PD-L1 antibody is conjugated to FITC
23
19
18
12
6
3
3
3
2
2
2
1
1
Application
Flow Cytometry (FACS)
125
117
81
44
16
16
15
8
6
4
3
3
2
2
2
2
2
2
2
1
1
Supplier
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Immunogen Mouse CD274 - transfected L5178Y cells
Clone MIH6
Isotype IgG2a
Specificity This antibody detects CD274, also known as B7-H1 and PD-1L.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Characteristics Synonyms: PD-L1, PDCD1 ligand 1, B7H1, B7 homolog 1, PDCD1L1, PDCD1LG1, Programmed cell death 1ligand 1, Programmed death ligand 1
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD274 / PDL1 (CD274 Antibody Abstract)
Background CD274 is a cell surface glycoprotein which is a member of the B7 family of co-stimulatory molecules. CD274 is expressed constitutively on macrophages and dendritic cells, and is induced on activated T-cells, B-cells, endothelial cells and epithelial cells in response to Interferons alpha, beta and gamma. CD274 is reported to possess dual functions, inhibition of activated effector T cells and co-stimulation of naive T cells. CD274 inhibits proliferation of activated T cells via ligation to the co-inhibitory molecule CD279 (programmed death-1, PD-1) leading to the secretion of the regulatory cytokine interleukin-10. CD274 has also been shown to costimulate early T cell priming and differentiation. Deregulated CD274 function has been reported in chronic viral and intracellular bacterial infection, as well as in many autoimmune diseases and cancers.Synonyms: B7 homolog 1, B7H1, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1, Programmed death ligand 1
Gene ID 60533
UniProt Q9EP73
Pathways
Application Notes Flow Cytometry: Use 10 μL of neat antibody to label 1x10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for anti-PD-L1 antibody (CD274 Molecule)  (FITC) (ABIN317020) anti-CD274 Molecule (CD274) antibody (FITC)
Background publications Kanai, Totsuka, Uraushihara et al.: "Blockade of B7-H1 suppresses the development of chronic intestinal inflammation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 8, pp. 4156-63, 2003 (PubMed).

Yamazaki, Akiba, Iwai et al.: "Expression of programmed death 1 ligands by murine T cells and APC." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 10, pp. 5538-45, 2002 (PubMed).