ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) antibody

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Antigen
  • MDR1
  • xemdr
  • ABC20
  • CD243
  • CLCS
  • GP170
  • P-GP
  • PGY1
  • p-gp
  • Abcb1
  • Mdr1a
  • ABCB1
  • PGP1
  • Mdr1
  • Mdr1b
  • Pgy-1
  • Pgy1
  • mdr
  • Mdr
  • Cd243
  • Mdr-1
  • Mod-2
  • Mod-2r
  • Mod-2s
  • Mod2-r
  • Mod2-s
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1A
  • ATP-binding cassette, sub-family B (MDR/TAP), member 1B
  • ATP-binding cassette, subfamily B (MDR/TAP), member 1B
  • malic enzyme complex, mitochondrial
  • ABCB1
  • abcb1
  • Abcb1a
  • Abcb1b
  • Mod2
Reactivity
Human, Monkey
268
28
18
13
10
8
7
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
181
98
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
14
9
9
7
5
5
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
141
81
74
74
37
34
23
22
8
7
6
3
3
3
2
2
1
Options
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Immunogen Mouse Balb/c 3T3 fibroblasts transfected with human CD243 cDNA.
Clone UIC2
Isotype IgG2a
Specificity This antibody specifically recognises an extracellular conformational epitope of CD243, also known as MDR1 (multidrug resistance protein 1) and Pgp (Pglycoprotein), a transmembrane protein and member of the ABC transporter (ATPbinding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. Clone UIC2 can be used in a shift assay to selectively demonstrate the expression and functional activity of CD243 in a target cell. Clone UIC2 does not cross-react with mitochondrial pyruvate carboxylase. We recommend the use of AM05632LE-N for Functional Studies.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Human, Primate.
Characteristics Synonyms: Multidrug resistance protein 1, P-glycoprotein 1, ABCB1, PGY1, ATP-binding cassettesub-family B member 1
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD243 / MDR1 (ABCB1 Antibody Abstract)
Background CD243 is also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein), a transmembrane protein and member of the ABC transporter (ATP-binding cassette) family, which acts as an active efflux pump for a diverse range of lipophillic compounds. CD243 is expressed at low levels in the cell membrane of peripheral blood leucocytes, and constitutively expressed on the apical plasma membrane of excretory epithelial cells of the kidney, liver, brain and small intestine. CD243 mediates resistance to many chemotherapeutic agents used for tumour suppression and is therefore of special interest to oncologists. Clone UIC2 is a strong inhibitor of CD243-mediated efflux and of the resistance of MDR cells to CD243 transported cytotoxic drugs.Synonyms: ABCB1, ATP-binding cassette sub-family B member 1, Multidrug resistance protein 1, P-glycoprotein 1, PGY1
Gene ID 5243
UniProt P08183
Application Notes Immunoprecipitation. Immunofluorescence. Flow Cytometry: Use 10 μL of neat MDR1/CD243 antibody to label 1x10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/200, This product may benefit from antigenretrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
Expiry Date 12 months
Background publications Mechetner, Roninson: "Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 13, pp. 5824-8, 1992 (PubMed).

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