ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2) antibody

Antigen: ATP-Binding Cassette, Sub-Family G (WHITE), Member 2 (ABCG2)

Synonyms: MRX, MXR, ABCP, BCRP, BMDP, MXR1, ABC15, BCRP1, CD338, CDw338, EST157481, MGC102821, Bcrp1, AI428558, ABCG2, MGC127688, bcrp

Clonality: Monoclonal (BXP-21)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN317260

Additional Information

Characteristics: Synonyms: Breast cancer resistance protein 1, ABCP, MXR, Mitoxantrone resistance-associatedprotein, Placenta-specific ATP-binding cassette transporter, ATP-binding cassettesub-family G member 2

Alternative name: CD338 / ABCG2 / BCRP1

Swiss-Prot: Q9UNQ0

Immunogen: Fusion protein consisting of the E. coli maltose binding protein and a 126 amino acid part ofthe CD338 peptide corresponding to aa 271-396 of BCRP according to Genebank accessionnumber AF103796. HPRD: 04783

Cross-Reactivity: Human

Isotype: IgG2a  (Matching secondary antibodies)

Clone: BXP-21

Description: An ABC transporter. Allows efflux of Hoechst dye, a property that has been used toseparate bone marrow side population cells, which express BCRP/ABCG2. Appears to playa major role in the multidrug resistance phenotype of a specific MCF-7 breast cancer cellline. When overexpressed, the transfected cells become resistant to mitoxantrone,daunorubicin and doxorubicin, display diminished intracellular accumulation ofdaunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123.

Specificity: This antibody recognises an internal epitope of CD338, otherwise known as the BreastCancer Resistance Protein (BCRP), a 70kDa transmembrane half transporter involved inmultidrug resistance. This antibody does not cross react with human MDR1, MRP1 or MRP2gene products. Species: Human. Others not tested.

Application Details

Application Notes: Immunohistochemistry on frozen sections. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.25 mg/ml

Buffer: Contains 0.09% Sodium Azide (NaN3), 0.7% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by

Restrictions: For Research Use only