IL12B antibody

Catalog No. ABIN317391

Product Details anti-IL12B Antibody

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Human, Sheep, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL12B antibody is un-conjugated

Application: Flow Cytometry (FACS), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: This antibody recognizes Interleukin-12. It has been shown to block the biological activity of IL-12.

Characteristics: Synonyms: Interleukin-12B, IL-12B, IL-12 subunit p40, CLMF p40, NKSF2, Cytotoxic lymphocytematuration factor 40 kDa subunit, NK cell stimulatory factor chain 2

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Recombinant bovine IL-12

Clone: CC326

Isotype: IgG2b

Application Details

Application Notes: ELISA: 1/100 - 1/1000. Flow cytometry: 1/1000, use 10 μL to label 10^6 cells of 100 l. Functional assays: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Preservative: Azide free

Storage: -20 °C

Storage Comment: Store the antibody undiluted at -20 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: Interleukin-12 beta / IL12B (IL12B Products)

Synonyms: CLMF antibody, CLMF2 antibody, IL-12B antibody, NKSF antibody, NKSF2 antibody, p40 antibody, Il-12b antibody, Il12p40 antibody, Il-12p40 antibody, Il12 antibody, IL-12p40 antibody, IL-12 antibody, IL-12 p40 antibody, il12b antibody, il12p40.b antibody, zgc:152789 antibody, IL-12.p40 antibody, IL12p40 antibody, IL12B antibody, CLMF p40 antibody, interleukin 12B antibody, interleukin 12b antibody, interleukin 12Ba antibody, IL12B antibody, Il12b antibody, il12ba antibody

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p40, Cytotoxic lymphocyte maturation factor 40 kDa subunit, IL-12 subunit p40, IL-12B, Interleukin-12B, NK cell stimulatory factor chain 2, NKSF2

Gene ID: 281857

UniProt: P46282

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation