LAMP1 antibody (FITC)

Catalog No. ABIN317423

Product Details anti-LAMP1 Antibody (FITC)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody is specific for Porcine CD107a, a cell surface antigen that is also known as lysosomal-associated membrane protein-1 (LAMP-1). CD107a is expressed on macrophages and more weakly on monocytes and granulocytes.

Cross-Reactivity (Details): Species reactivity (tested):Pig.

Characteristics: Synonyms: LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, CD107Antigen-like Family Member A

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Porcine alveolar Macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouseX63-Ag. 8. 653 myeloma cell line.

Clone: 4E9-11

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of Neat antibody to label 10^6 cells in 100 μL (Membranepermeabilisation is required).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: CD107a / LAMP1 (LAMP1 Products)

Synonyms: CD107a antibody, LAMPA antibody, LGP120 antibody, AI196048 antibody, Lamp-1 antibody, LEP100 antibody, LAMP-1 antibody, LGP-A antibody, Lgpa antibody, Lysosome-associated membrane protein 1 antibody, cd107a antibody, lamp-1 antibody, lamp1 antibody, lampa antibody, lgp120 antibody, rexp76 antibody, lysosomal associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 L homeolog antibody, LAMP1 antibody, Lamp1 antibody, lamp1.L antibody

Background: Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.Synonyms: CD107 Antigen-like Family Member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1

Gene ID: 494565

UniProt: Q5K4G2

Pathways: Autophagy