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LAMP2 antibody (Lysosomal-Associated Membrane Protein 2) (FITC)

Details for Product anti-LAMP2 Antibody No. ABIN317431, Supplier: Log in to see
Antigen
  • cd107b
  • lamp-2
  • lampb
  • LAMP2
  • lamp2
  • DKFZp459J126
  • LAMP-2
  • LGP-B
  • CD107b
  • Lamp-2
  • Lamp-2a
  • Lamp-2b
  • Lamp-2c
  • Mac3
  • LAMPB
  • LGP110
Reactivity
Dog (Canine), Human, Mink
241
176
32
24
18
5
Host
Mouse
153
117
115
1
Clonality (Clone)
Monoclonal ()
Conjugate
This LAMP2 antibody is conjugated to FITC
51
36
27
22
10
10
6
5
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Flow Cytometry (FACS)
192
149
99
90
77
70
63
37
36
11
9
8
4
4
2
2
2
1
1
1
1
1
Supplier
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Immunogen MDCK (Madin-Darby Canine Kidney) cells
Clone AC17
Isotype IgG1
Specificity This antibody recognises canine CD107b, otherwise known as LAMP-2, a 95 kDa heavily-glycosylated type I integral lysosomal glycoprotein, shown to share high N-terminal amino acid sequence homology with human, mouse and rat CD107b.
Characteristics Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD107b / LAMP2 (LAMP2 Antibody Abstract)
Background Lysosomes are membrane-bound organelles found within the cytoplasm of most cells, which contain hydrolytic enzymes and act as the major compartment for heterophagic and autophagic digestion. Members of the lysosomal-associated membrane protein family (LAMPS) are believed to play an important role in protecting the lysosomal membrane from protease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins and the endocytic pathway and human studies have revealed a correlation between the level of surface expression of CD107b on tumour cells and their metastatic potential.Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Gene ID 3920
UniProt P13473
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways
Application Notes Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required. Clone AC17 has been shown as suitable for use in Electron Microscopy (1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer Phosphate buffered saline pH 7.4, 0.09 % Sodium Azide (NaN3), 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Ihrke, Bruns, Luzio et al.: "Competing sorting signals guide endolyn along a novel route to lysosomes in MDCK cells." in: The EMBO journal, Vol. 20, Issue 22, pp. 6256-64, 2001 (PubMed).

Jou, Leung, Fung et al.: "Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1." in: Molecular biology of the cell, Vol. 11, Issue 1, pp. 287-304, 2000 (PubMed).

Nabi, Rodriguez-Boulan: "Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer." in: Molecular biology of the cell, Vol. 4, Issue 6, pp. 627-35, 1993 (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." in: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, 1991 (PubMed).

Nabi, Le Bivic, Fambrough et al.: "An endogenous MDCK lysosomal membrane glycoprotein is targeted basolaterally before delivery to lysosomes." in: The Journal of cell biology, Vol. 115, Issue 6, pp. 1573-84, 1992 (PubMed).