Lysosomal-Associated Membrane Protein 2 (LAMP2) antibody

Details for Product No. ABIN317432
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Synonyms cd107b, lamp-2, lampb, LAMP2, lamp2, DKFZp459J126, LAMP-2, LGP-B, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3, LAMPB, LGP110
Mink, Dog (Canine), Human
(126), (118), (24), (20), (13), (13), (7), (1)
(90), (86), (53), (1)
Clonality (Clone)
Monoclonal ()
(32), (22), (11), (11), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunoelectron Microscopy (IEM), Flow Cytometry (FACS)
(102), (99), (59), (53), (48), (41), (30), (30), (28), (10), (2), (1)
Pubmed 5 references available
Quantity 0.25 mg
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Catalog No. ABIN317432
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Immunogen MDCK (Madin-Darby Canine Kidney) cells. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cellline.
Clone AC17
Isotype IgG1
Characteristics Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Purification Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Alternative Name CD107b / LAMP2
Background Lysosomes are membrane-bound organelles found within the cytoplasm of most cells,which contain hydrolytic enzymes and act as the major compartment for heterophagic andautophagic digestion. Members of the lysosomal-associated membrane protein family(LAMPS) are believed to play an important role in protecting the lysosomal membrane fromprotease degradation and are involved in lectin-mediated cell adhesion. Evidence shows that CD107b is involved in the lysosomal uptake of cytosolic proteins andthe endocytic pathway and human studies have revealed a correlation between the level ofsurface expression of CD107b on tumour cells and their metastatic potential.
UniProt P13473
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry (Membrane permeabilisation is required). Immunoprecipitation. Western blot: 1/100-1/500. Immunofluorescence. Clone AC17 has been shown as suitable for use in Electron Microscopy (See Ref.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Nabi, Le Bivic, Fambrough et al.: "An endogenous MDCK lysosomal membrane glycoprotein is targeted basolaterally before delivery to lysosomes." in: The Journal of cell biology, Vol. 115, Issue 6, pp. 1573-84, 1992 (PubMed).

Fukuda: "Lysosomal membrane glycoproteins. Structure, biosynthesis, and intracellular trafficking." in: The Journal of biological chemistry, Vol. 266, Issue 32, pp. 21327-30, 1991 (PubMed).

Nabi, Rodriguez-Boulan: "Increased LAMP-2 polylactosamine glycosylation is associated with its slower Golgi transit during establishment of a polarized MDCK epithelial monolayer." in: Molecular biology of the cell, Vol. 4, Issue 6, pp. 627-35, 1993 (PubMed).

Jou, Leung, Fung et al.: "Selective alterations in biosynthetic and endocytic protein traffic in Madin-Darby canine kidney epithelial cells expressing mutants of the small GTPase Rac1." in: Molecular biology of the cell, Vol. 11, Issue 1, pp. 287-304, 2000 (PubMed).

Ihrke, Bruns, Luzio et al.: "Competing sorting signals guide endolyn along a novel route to lysosomes in MDCK cells." in: The EMBO journal, Vol. 20, Issue 22, pp. 6256-64, 2001 (PubMed).

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