Prothrombin antibody
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- Target See all Prothrombin (F2) Antibodies
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Prothrombin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of Prothrombin protein.(region surrounding Gly724)
- Purification
- Affinity Chromatography using epitope-specific immunogen.
- Top Product
- Discover our top product F2 Primary Antibody
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- Application Notes
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Western Blot: 1/500-1/1000. ELISA: 1/10000-1/20000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))
- Alternative Name
- Prothrombin (F2) (F2 Products)
- Synonyms
- PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, zgc:66319 antibody, wu:fb57c10 antibody, wu:fd42e09 antibody, wu:fd59d01 antibody, prothrombin antibody, F2 antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, coagulation factor II (thrombin) antibody, F2 antibody, f2 antibody
- Background
- Hemostasis following tissue injury involves the deployment of essential plasma procoagulants (Prothrombin and Factors X, IX, V and VIII), which mediate a blood coagulation cascade that leads to the formation of insoluble Fibrin clots and the promotion of platelet aggregation. Proteolytic cleavage of Prothrombin (Factor II) at residue 44 leads to formation of Thrombin in the first step of the coagulation cascade. Thrombin cleaves bonds between Arg and Gly and activates Factors V, VII, VIII and XIII in complex with thrombomodulin and Protein C. Thrombin maintains vascular integrity during development and postnatal life and coordinates connective tissue proteins by stimulating fibroblast procollagen production.Synonyms: Coagulation factor II
- Molecular Weight
- approx. 70 kDa
- Gene ID
- 2147
- NCBI Accession
- NP_000497
- UniProt
- P00734
- Pathways
- Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling
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