RAD51D antibody

Catalog No. ABIN317685

Product Details anti-RAD51D Antibody

Target: RAD51D (RAD51 Homolog D (RAD51D))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51D antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of Rad51D protein.(region surrounding Ala152)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000-1/20000. Western Blot: 1/500-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2 containing 0,05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAD51D

Target: RAD51D (RAD51 Homolog D (RAD51D))

Alternative Name: RAD51L3 (RAD51D Products)

Synonyms: R51H3 antibody, Rad51l3 antibody, TRAD antibody, RAD51 paralog D antibody, Rad51d antibody

Background: Rad52 family members (Rad50, Rad51B/C/D, Rad52, Rad54, MRE11) mediate DNA double-strand break repair (DSBR) for DNA damage that otherwise could cause cell death, mutation or neoplastic transformation. Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer.Synonyms: DNA repair protein RAD51 homolog 4, R51H3, RAD51 homolog D, RAD51-like protein 3, RAD51D, TRAD

Molecular Weight: approx. 40 kDa

Gene ID: 5892

NCBI Accession: NP_001136043

UniProt: O75771