CHRM1 antibody

Catalog No. ABIN317696

Product Details anti-CHRM1 Antibody

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHRM1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects endogenous levels of Muscarinic acetylcholine receptor M1 / mAChR M1 protein (region surrounding Arg336).

Purification: Affinity-chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHRM1

Target: CHRM1 (Muscarinic Acetylcholine Receptor M1 (CHRM1))

Alternative Name: Muscarinic Acetylcholine Receptor M1 (CHRM1 Products)

Synonyms: M1 antibody, CHRM1 antibody, HM1 antibody, M1R antibody, mAChR antibody, Chrm-1 antibody, cholinergic receptor muscarinic 1 antibody, cholinergic receptor, muscarinic 1 antibody, cholinergic receptor, muscarinic 1, CNS antibody, CHRM1 antibody, Chrm1 antibody

Background: The muscarinic acetylcholine receptors (mAChR) mediate a variety of cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels. The mAChRs transducer signals by coupling to G-proteins, which then modulate several downstream effector proteins and ion channels. Five mAChR subtypes have been identified, designated M1 to M5. The five receptor subtypes show distinct patterns of tissue distribution, as well as distinct pharmacological and functional properties. The amino acid sequence of each mAChR subtype reflects a structure that is characteristic of G-protein coupled receptors, consisting of seven highly conserved transmembrane segments and a large intracellular region unique to each subtype, which constitutes the effector-coupling domain.Synonyms: CHRM1, mAChR M1, mAChR-M1

Molecular Weight: Approx. 50 kDa

Gene ID: 1128

NCBI Accession: NP_000729

UniProt: P11229

Pathways: Synaptic Membrane