MC4R antibody

Catalog No. ABIN317754

Product Details anti-MC4R Antibody

Target: MC4R (Melanocortin 4 Receptor (MC4R))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MC4R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: MC4-R antibody detects endogenous levels of MC4-R protein.(region surrounding Glu315)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MC4R

Target: MC4R (Melanocortin 4 Receptor (MC4R))

Alternative Name: MC4-R (MC4R Products)

Synonyms: zMC4 antibody, si:dkey-151m6.62 antibody, mc4r antibody, MC4R antibody, LOC100218148 antibody, Mc4-r antibody, melanocortin 4 receptor antibody, mc4r antibody, MC4R antibody, Mc4r antibody

Background: MC4 receptor is the fourth member of the five known melanocortin receptors (MCRs) belonging to the rhodopsin-like family of G protein-coupled receptors (GPCRs). These five MCRs form a unique family of GPCRs, marked by their unusually short coding sequence and the absence of highly conserved amino acid residues or motifs common to most GPCRs. All MCRs signal through the cyclic adenosine 3', 5'-monophosphate (cAMP) pathway. With the exception of MC2R, all MCRs are activated by both adrenocorticotropin (ACTH) and melanocyte-stimulating hormones (MSHs and mainly alpha-MSH). The MC4R receptor is expressed primarily in the brain, and unlike the other melanocortin receptors, MC4R is not expressed in adrenal cortex or in melanocytes. Genetic studies of mice and humans have established a critical role of MC4 Receptor in appetite regulation. Heterozygous mutations in MC4 Receptor account for 1-6 % of severe cases of human obesity.Synonyms: MC4R, Melanocortin receptor 4

Molecular Weight: approx. 37 kDa

Gene ID: 4160

NCBI Accession: NP_005903

UniProt: P32245

Pathways: Hormone Transport, cAMP Metabolic Process, Feeding Behaviour