CYP7B1 antibody

Catalog No. ABIN317809

Product Details anti-CYP7B1 Antibody

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP7B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of CYP7B1 protein.(region surrounding Gln127)

Purification: Affinity-Chromatography using epitope-specific immunogen

Application Details

Application Notes: ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH ~7.2 with 0.05 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CYP7B1

Target: CYP7B1 (Cytochrome P450, Family 7, Subfamily B, Polypeptide 1 (CYP7B1))

Alternative Name: CYP7B1 (CYP7B1 Products)

Synonyms: CYP7B1 antibody, CBAS3 antibody, CP7B antibody, SPG5A antibody, AW261589 antibody, D3Ertd552e antibody, hct-1 antibody, cytochrome P450 family 7 subfamily B member 1 antibody, cytochrome P450, family 7, subfamily B, polypeptide 1 antibody, cytochrome P450, family 7, subfamily b, polypeptide 1 antibody, CYP7B1 antibody, cyp7b1 antibody, Cyp7b1 antibody

Background: P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds including cholesterol. CYP8B1 moderates the ratio of cholic acid over chenodeoxycholic acid to control the solubility of cholesterol. P450 cholesterol 7-hydroxylase, CYP7A1, is the rate limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. CYP7B1 (oxysterol 7-α-hydroxylase) functions as an enzyme in the alternate bile acid synthesis pathway. Specifically, CYP7B1 metabolizes 25-and 27-hydroxycholesterol. The gene encoding human CYP7B1 maps to chromosome 8q21.3. Mutations in the CYP7B1 gene may cause a metabolic defect in bile acid synthesis characterized by elevated urinary bile acid excretion, severe cholestasis, cirrhosis and liver synthetic failure.Synonyms: 25-hydroxycholesterol 7-alpha-hydroxylase, Cytochrome P450 7B1, Oxysterol 7-alpha-hydroxylase

Molecular Weight: approx. 58 kDa

Gene ID: 9420

NCBI Accession: NP_004811

UniProt: O75881

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Biosynthesis, Regulation of Intracellular Steroid Hormone Receptor Signaling