GNL1 antibody

Catalog No. ABIN317914

Product Details anti-GNL1 Antibody

Target: GNL1 (Guanine Nucleotide Binding Protein Like Protein 1 (GNL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNL1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: GNL1 antibody detects endogenous levels of GNL1 protein.(region surrounding Gln103)

Purification: Affinity chromatography

Application Details

Application Notes: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GNL1

Target: GNL1 (Guanine Nucleotide Binding Protein Like Protein 1 (GNL1))

Alternative Name: GNL1 / HSR1 (GNL1 Products)

Synonyms: HSR1 antibody, Gna-rs1 antibody, Gnal1 antibody, G protein nucleolar 1 (putative) antibody, guanine nucleotide binding protein-like 1 antibody, G protein nucleolar 1 antibody, GNL1 antibody, Gnl1 antibody

Background: GNL1 (guanine nucleotide-binding protein-like 1) is a nuclear protein that likely acts as a regulator of the histocompatibility cluster. GNL1 and MMR1, the murine homolog, are localized within or close to the MHC class I region and belong to the MMR1/HSR1 GTP-binding protein family. GTPases, such as GNL1, from the MMR1/HSR1 GTP-binding protein subfamily are circularly rearranged G-motifs that play a critical role in maintaining normal cell growth. Deletion of these genes results in severe growth defects with a marked reduction in mature rRNA species and a concomitant accumulation of the 35S pre-rRNA transcript. Deletion also causes the ribosomal protein RPL25A to fail exportation from the nucleolus. Deletion of any of the G-domain motifs will result in a null phenotype and nuclear/nucleolar localization that lacks the nucleolar export of preribosomes and is accompanied by a distortion of the nucleolar structure. Upon DNA damage GNL1 is phosphorylated by a kinase, possibly Atm or ATR.Synonyms: GTP-binding protein HSR1, Guanine nucleotide-binding protein-like 1

Molecular Weight: approx. 69 kDa

Gene ID: 2794

NCBI Accession: NP_005266

UniProt: P36915