Leptin antibody (AA 57-70)

Catalog No. ABIN318591

Product Details anti-Leptin Antibody

Target: Leptin (LEP)

Binding Specificity: AA 57-70

Reactivity: Human, Mouse, Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Specificity: This antibody recognises leptin.

Purification: Purified

Immunogen: HMW conjugated leptin amino acids 57-70.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/50,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5.0mg/mL

Buffer: Phosphate buffered saline pH 7.4 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store at +4 °C or at -20 °C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted.

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin (LEP Products)

Synonyms: ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Background: Leptin is a 16 kDa secreted protein hormone encoded by the OB gene, which plays a key role in the regulation of metabolism, body weight and reproduction and is expressed primarily by white adipocytes, and to a lesser extent by stomach endothelium and the placenta. Regulation of food intake and energy expenditure by leptin is thought to predominantly occur in the hypothalamus of the brain, where leptin binds with high affinity to neuronal leptin receptors (OB-R), inhibiting the action of the feeding stimulants neuropeptide Y (NPY) and agouti-related peptide (AgRP), or promoting the effects of the appetite regulator alpha-melanocyte-stimulating hormone (alpha-MSH). Leptin also acts as a direct stimulator of fatty acid oxidation in the mitochondria of liver and skeletal muscle cells, and influences glucose and fat metabolism. The expression of leptin in relation to metabolic status is influenced by hormonal and metabolite control: stimulators of leptin include insulin and cortisol, whilst cAMP, thiazolidinediones and beta-adrenergic receptor agonists, reduce leptin expression.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gene ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process