ERN1 antibody (C-Term)

Catalog No. ABIN318702

Product Details anti-ERN1 Antibody

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises inositol-requiring kinase 1 precursor (IRE1p).

Purification: Affinity Chromatography

Immunogen: A 16 amino acid peptide near the carboxy terminus of Human IRE1p

Isotype: IgG

Application Details

Application Notes: Western blot: 1 - 2 μg/mL (detects a band of approximately 110 kDa in A-20 cell lysate).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: ERN1 / IRE1 (ERN1 Products)

Synonyms: IRE1 antibody, IRE1P antibody, IRE1a antibody, hIRE1p antibody, 9030414B18Rik antibody, AI225830 antibody, C85377 antibody, Ire1a antibody, Ire1alpha antibody, Ire1p antibody, RGD1559716 antibody, Ern1 antibody, Ire1 antibody, Ire1b antibody, sb:eu1005 antibody, zgc:109932 antibody, endoplasmic reticulum to nucleus signaling 1 antibody, endoplasmic reticulum to nucleus signaling 2 antibody, endoplasmic reticulum (ER) to nucleus signalling 1 antibody, serine/threonine-protein kinase/endoribonuclease IRE1 antibody, endoplasmic reticulum (ER) to nucleus signalling 2 antibody, ERN1 antibody, ERN2 antibody, Ern1 antibody, LOC524719 antibody, Ern2 antibody, ern1 antibody

Background: Inositol-requiring kinase 1 precursor (IRE1p) is a 108 kDa serine-threonine kinase/endoribonuclease also known as ERN1. It is an endoplasmic reticulum (ER) transmembrane protein, normally bound to the ER molecular chaperones GRP78 and GRP94. At times of ER stress, due to accumulation of unfolded proteins, IRE1p becomes dissociated from the chaperones, which allows it to activate the un-folded protein transcriptional response (UPR). In the mammalian genome, there are two homologs Ire1alpha and Ire1beta. Ire1alpha is expressed in all cells and tissues, Ire1beta expression is primarily restricted to intestinal epithelial cells.Synonyms: Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, IRE1p, Inositol-requiring protein 1, Ire1-alpha, Serine/threonine-protein kinase/endoribonuclease IRE1

Gene ID: 2081

NCBI Accession: NP_001424

UniProt: O75460

Pathways: ER-Nucleus Signaling, Unfolded Protein Response