tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE) (pSer58) antibody

Antigen: tyrosine 3-Monooxygenase/tryptophan 5-Monooxygenase Activation Protein, epsilon Polypeptide (YWHAE)

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517

Epitope: pSer58

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Rabbit, Rat (Rattus), Sheep (Ovine)

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN318720

Additional Information

Characteristics: Synonyms: 14-3-3E, YWHAE

Alternative name: 14-3-3 protein epsilon

Swiss-Prot: P62258

Immunogen: Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3epsilonAA Sequence: Ac. MDDREDLVYQAKC

Format: Serum

Isotype: IgG  (Matching secondary antibodies)

Description: 14-3-3 epsilon is a member of the 14-3-3 family which consists of 30 kDa proteins that areinvolved in multiple protein kinase signalling pathways, regulation of cell cycleprogression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and areregulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancersdisrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon isthought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also apotential suicide susceptibility gene.

Specificity: This antibody recognises the acetylated N-terminal of 14-3-3 epsilon. The antibody may not react with recombinant proteins that are not N-acetylated. Species: Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep. Others not tested.

Synonyms: MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465, AU019196, 14-3-3e, 14-3-3, 14-3-3-e, CT24092, D14-3-3e, D14-3-3epsilon, EK3-5, PAR5, Par-5, SR3-9, Su(Raf)3B, anon-WO0172774.141, anon-WO02059370.52, l(3)j2B10, DmelCG31196, CG31196, LOC443300, YWHAE, mds, mdcr, kcip-1, MGC89915, 14-3-3epsilon, LOC581376, Ywhae, ACYPI002517

Application Details

Application Notes: Immunohistochemistry on paraffin sections (requires antigen retrieval using heattreatment prior to staining of paraffin sections). ELISA. Western blot. Histology Positive Control Tissue: Normal brain tissue. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: 0.09% Sodium Azide (NaN3)

Storage: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use. Shelf life: one year from despatch.

Research Area: Transcription Factors

Restrictions: For Research Use only