14-3-3 Pan antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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| Catalog no. |
ABIN318725 |
| Quantity |
0.1 ml (Variants) |
| Price |
450.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: YWHA, Protein kinase C inhibitor protein 1
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Alternative name
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14-3-3 protein pan
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Immunogen
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Synthetic correspsonding to all isoforms of sheep 14-3-3.
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Cross-Reactivity
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Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processesincluding protein kinase signalling pathways, regulation of cell cycle progression,transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinctpreferences for its different isoforms and are regulated by phosphorylation of both 14-3-3and the bound protein.
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Specificity
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This antibody recognises all isoforms of the 14-3-3 protein family. Species: Sheep, Human, Bovine, Rat, Mouse. Others not tested.
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Application Notes
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Immunohistochemistry on paraffin sections: does not require antigen retrieval using heattreatment prior to staining of paraffin sections. Immunoprecipitation. Western Blot: detects a band of approximately 30kDa in HEK293 cell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Purification
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Serum
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.
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Research Area
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Transcription Factors
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Restrictions
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For Research Use only
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Alternatives for antigen "14-3-3 Pan", type "Antibodies"
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Hosts
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Rabbit (5), Mouse (1), Sheep (1)
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Reactivities
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Cow (Bovine) (5), Human (5), Chicken (3), Dog (Canine) (3), Mouse (Murine) (3), Rat (Rattus) (3), Xenopus laevis (3), Monkey (2), Sheep (Ovine) (2), Primate (1)
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Applications
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Western Blotting (WB) (5), Immunofluorescence (IF) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)
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Epitopes
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pSer58 (3), C-Term (1)
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