Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (N-Term) antibody

Details for Product No. ABIN318734
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Synonyms darpp32, darpp-32, Darpp-32, Darpp32, DARPP-32, DARPP32, AU040756
(44), (32), (11), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(134), (117), (102), (50), (48), (36), (36), (11), (4), (3), (3), (1), (1), (1)
(144), (8), (2), (2), (1)
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Western Blotting (WB)
(118), (57), (46), (30), (25), (20), (9), (4), (4), (2), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mL
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Catalog No. ABIN318734
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Immunogen Peptide corresponding to the N-terminal region of rat DARPP-32, conjugated to keyhole limpet haemocyanin (KLH)
Isotype IgG
Specificity This antibody recognises DARPP-32 at N-term. Detects a band of approximately 32kDa in rat hippocampal lysate.
Purification Affinity chromatography
Alternative Name DARPP32
Background DARPP-32 is a 32 kDa protein phosphatase which modulates cell signalling in neurons. It is converted into an inhibitor of protein phosphatase-1 when it is phosphorylated by protein kinase A as a result of dopaminergic receptor stimulation. However this phosphorylation is reversed on stimulation of N-methyl-D-aspartate (NMDA) receptors by glutamate. Conversely when phosphorylated at threonine-75 by CDK5, DARPP-32 becomes an inhibitor of protein kinase A. DARPP-32 has been linked to gastric cancer. The phosphatase is highly expressed in the nucleus accumbens, caudate nucleus, cerebellar cortex and putamen, being expressed at lower levels in the neocortical regions.
Alternate names: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
Gene ID 84152
NCBI Accession NP_001229393
UniProt Q9UD71
Research Area Signaling
Application Notes Western blot: 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM Hepes pH 7.5, 0.09% Sodium Azide, 0.01% Bovine Serum Albumin, 50% Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for up to one month or at -20°C for longer.
Expiry Date 12 months
Background publications Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

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