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ORAI1 antibody (ORAI Calcium Release-Activated Calcium Modulator 1) (C-Term)

Details for Product anti-ORAI1 Antibody No. ABIN318921, Supplier: Log in to see
Antigen
  • im:7146264
  • orai1
  • tmem142a
  • zgc:109721
  • orai2
  • ORAI1
  • LOC100218031
  • CRACM1
  • ORAT1
  • TMEM142A
  • D730049H07Rik
  • Tmem142a
  • orai-1
  • RGD1311873
  • tmem142b
Epitope
C-Term
52
21
16
15
11
10
3
3
2
2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
117
39
25
4
4
4
3
1
1
Host
Rabbit
83
20
14
Clonality
Polyclonal
Conjugate
This ORAI1 antibody is un-conjugated
5
5
5
5
5
5
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
106
79
44
12
6
4
1
Supplier
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Immunogen A 16 amino acid peptide from near the carboxy terminus of Human ORAI1.
Isotype IgG
Specificity This antibody recognises ORAI1, also known as Transmembrane protein 142A (TMEM142A), a 32.7 kDa multi-pass membrane protein belonging to the ORAI family. This antibody is not expected to cross react with either ORAI2 or ORAI3.
Purification Affinity Chromatography
Alternative Name ORAI1 (ORAI1 Antibody Abstract)
Background Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI1 is a four- transmembrane spanning protein that is an essential component of CRAC. ORAI1 functions as a highly selective Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Defective ORAI1 in humans is the cause of one form of hereditary severe combined immune deficiency (SCID), which results in ablated T-cell Ca2+ entry.Synonyms: CRACM1, Calcium release-activated calcium channel protein 1, ORAI-1, Protein orai-1, TMEM142A, Transmembrane protein 142A
Gene ID 84876
NCBI Accession NP_116179
UniProt Q96D31
Application Notes Western Blot: 0.5 - 1g/mL, detects a band of approximately 49 kDa in 49 cell lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-ORAI1 antibody (ORAI Calcium Release-Activated Calcium Modulator 1) (C-Term) (ABIN318921) anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibody
 image for anti-ORAI1 antibody (ORAI Calcium Release-Activated Calcium Modulator 1) (C-Term) (ABIN318921) anti-ORAI Calcium Release-Activated Calcium Modulator 1 (ORAI1) (C-Term) antibody (Image 2)
Background publications Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Lewis: "Calcium signaling mechanisms in T lymphocytes." in: Annual review of immunology, Vol. 19, pp. 497-521, 2001 (PubMed).