14-3-3 Pan antibody

Antigen: 14-3-3 Pan

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Western Blotting (WB)

Catalog no.: ABIN318952

Additional Information

Characteristics: Synonyms: YWHA, Protein kinase C inhibitor protein 1

Alternative name: 14-3-3 protein pan

Immunogen: Peptide corresponding to amino acid residues from the C-terminal region of the 14-3-3protein, conjugated to keyhole limpet hemocyanin (KLH).

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: 14-3-3 proteins form homo- and heterodimers that can interact with a variety of cellularproteins that are typically phosphorylated on serine or threonine residues. 14-3-3 proteinsregulate cellular processes such as trafficking, cell-cycle regulatory pathways and signaltransduction. Brain tissue contains the highest concentration of 14-3-3 proteins and theproteins have been found in the cerebral spinal fluid of patients with certainneurodegenerative disease such as Creutzfeldt-Jakob disease and scrapie. 14-3-3 proteinsmight also contribute to Alzheimers and Parkinsons diseases, MillerDieker syndrome andspinocerebellar ataxia type 1. 14-3-3 has also been implicated in cancer.

Specificity: This antibody recognises 14-3-3 protein, a highly conserved, ubiquitously expressedprotein family. Species: Rat. Others not tested.

Application Details

Application Notes: Western Blot: 1: 1000, detects a band of approximately 29kDa in rat hippocampal lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 10mM Hepes pH7.5, 150mM NaCl containing 0.09% Sodium Azide (NaN3),50% Glycerol 0.01% Bovine Serum Albumin

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Research Area: Transcription Factors

Restrictions: For Research Use only