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CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term)

Details for Product anti-CFLAR Antibody No. ABIN318973, Supplier: Log in to see
Antigen
  • Clarp1
  • cflar
  • CFLAR
  • FLIP
  • CASH
  • CASP8AP1
  • CLARP
  • Casper
  • FLAME
  • FLAME-1
  • FLAME1
  • I-FLICE
  • MRIT
  • c-FLIP
  • c-FLIPL
  • c-FLIPR
  • c-FLIPS
  • 2310024N18Rik
  • A430105C05Rik
  • Cash
  • ENSMUSG00000072980
  • Flip
  • Gm9845
  • c-Flip
Alternatives
anti-Human CFLAR antibody for Immunofluorescence
Epitope
C-Term, AA 191-209
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Reactivity
Human, Mouse (Murine)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This CFLAR antibody is un-conjugated
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Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
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2
1
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Supplier
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Immunogen A peptide corresponding to aa191 - 209 at the C-terminus of human FLIP delta/FLIPs form.
Isotype IgG
Specificity This antibody recognises the apoptosis related protein designated FLIP (FLICE inhibitory protein).
Purification Purified
Alternative Name CFLAR / Casper / I-FLICE (CFLAR Antibody Abstract)
Background FLIP interacts with the adaptor protein FADD, and with Caspase-8 (FLICE) and Caspase-10 (FLICE2) to inhibit apoptosis induced by death receptors including CD95, DR3, TRAIL-R and TNFR1. FLICE inhibitory proteins were initially identified in virus and human and were accordingly designated as v-FLIPs and c-FLIPs. Four spice variants of c-FLIP have been identified and are classified as FLIP alpha, beta, delta and gamma. AHP1591 recognizes human and murine delta and gamma variants but not the alpha or beta, variants. FLIP is widely expressed in human tissues and a diverse range of cell lines.Synonyms: CASH, CASP8 and FADD-like apoptosis regulator, CASP8AP1, CLARP, Caspase homolog, Caspase-eight-related protein, Caspase-like apoptosis regulatory protein, Cellular FLICE-like inhibitory protein, FADD-like antiapoptotic molecule 1, FLAME-1, Inhibitor of FLICE, MACH-related inducer of toxicity, MRIT, Usurpin, c-FLIP
Gene ID 8837
NCBI Accession NP_001120655
UniProt O15519
Pathways Apoptosis
Application Notes Immunohistochemistry on frozen sections: 2g/mL. Western Blot: 1: 1000, detects bands of approximately 35 kDa and 25 kDa in Jurkat celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline containing 0.02 % Sodium Azide (NaN3)
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN318973) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209), (C-Term) antibody
 image for anti-CFLAR antibody (CASP8 and FADD-Like Apoptosis Regulator) (C-Term) (ABIN318973) anti-CASP8 and FADD-Like Apoptosis Regulator (CFLAR) (AA 191-209), (C-Term) antibody (Image 2)
Background publications Irmler, Thome, Hahne et al.: "Inhibition of death receptor signals by cellular FLIP." in: Nature, Vol. 388, Issue 6638, pp. 190-5, 1997 (PubMed).

Hu, Vincenz, Ni et al.: "I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis." in: The Journal of biological chemistry, Vol. 272, Issue 28, pp. 17255-7, 1997 (PubMed).

Shu, Halpin, Goeddel: "Casper is a FADD- and caspase-related inducer of apoptosis." in: Immunity, Vol. 6, Issue 6, pp. 751-63, 1997 (PubMed).

Thome, Schneider, Hofmann et al.: "Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors." in: Nature, Vol. 386, Issue 6624, pp. 517-21, 1997 (PubMed).