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tyrosine Hydroxylase (TH) (pSer31) antibody

Details for Product No. ABIN319110, Supplier: Log in to see
Antigen
  • CG10118
  • DH65B
  • DTH
  • Dmel\\CG10118
  • Pale
  • Ple
  • TH
  • Th
  • VII
  • dTH1
  • dTH65B
  • pale/ple
  • th
  • 14-3-3
  • 14-3-3 epsilon
  • 14-3-3-e
  • 14-3-3-epsilon
  • 14-3-30epsilon
  • 14-3-3EPSILON
  • 14-3-3e
  • 14-3-3omicron
  • 1433epsilon
  • 143E_DROME
  • CG31196
  • CT24092
  • D14-3-3e
  • D14-3-3epsilon
  • Dmel\\CG31196
  • EK3-5
  • PAR-5
  • PAR5
  • Par-5
  • SR3-9
  • Su(Raf)3B
  • anon-WO0172774.141
  • anon-WO02059370.52
  • d14-3-3epsilon
  • eps
  • l(3)j2B10
  • par-5
  • DYT14
  • DYT5b
  • TYH
  • The
  • pale
  • tyrosine 3-monooxygenase
  • CG31196 gene product from transcript CG31196-RB
  • tyrosine hydroxylase
  • ple
  • th
  • Tsp_12013
  • 14-3-3epsilon
  • TH
  • Th
Alternatives
anti-Mouse (Murine) tyrosine Hydroxylase antibody for Immunofluorescence
Epitope
pSer31
55
48
37
32
27
16
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Monkey, Mouse (Murine), Rat (Rattus)
318
276
175
30
24
18
12
11
6
6
4
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
250
108
17
15
8
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
5
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
336
199
195
94
94
69
62
53
36
13
10
7
5
4
4
3
2
1
Options
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Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes phosphorylated Ser31.
Isotype IgG
Specificity This antibody is specific for Tryosine Hydroxylase, when phosphorylated at serine 31.
Purification Affinity chromatography
Alternative Name Tyrosine 3-Monooxygenase (TH) (TH Antibody Abstract)
Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines, dopamine (DA), norepinephrine and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Phosphorylation of TH has been shown to occur at several serine residues, including serine 8, 19, 31 and 40. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson.Synonyms: TYH, Tyrosine 3-hydroxylase
Gene ID 25085
NCBI Accession NP_036872
UniProt P04177
Research Area Amino Acids, Enzymes
Pathways Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis
Application Notes Immunohistochemistry on frozen sections: 1: 1000. Western Blot: 1: 1000, detects a band of approximately 60 kDa in rat PC-12 cell lysates,following stimulation by Okadaic acid. Immunofluorescence: 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES, pH 7.5 containing 0.09 % Sodium Azide, 0.01 % Bovine Serum Albumin and 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Bevilaqua, Graham, Dunkley, von Nagy-Felsobuki, Dickson: "Phosphorylation of Ser(19) alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser(40)." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40411-6, 2001 (PubMed).

Lew, Garcia-Espana, Lee, Carr, Goldstein, Haycock, Meller: "Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity." in: Molecular pharmacology, Vol. 55, Issue 2, pp. 202-9, 1999 (PubMed).

Haycock: "Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 11682-91, 1990 (PubMed).