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tyrosine Hydroxylase (TH) (pSer31) antibody

Details for Product No. ABIN319110
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Antigen
Synonyms TYH, DYT14, DYT5b, The, CG10118, DH65B, DTH, Dmel\\CG10118, Pale, Ple, TH, Th, VII, dTH1, dTH65B, pale/ple, th
Epitope
pSer31
(44), (34), (33), (18), (16), (5), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Monkey, Mouse (Murine), Rat (Rattus)
(191), (163), (124), (16), (15), (14), (10), (4), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(225), (37), (11), (8), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(225), (126), (119), (79), (51), (44), (41), (30), (21), (4), (4), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Catalog No. ABIN319110
819.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic phosphopeptide corresponding to an amino acid sequence within Tyrosine Hydroxylase which includes phosphorylated Ser31.
Isotype IgG
Specificity This antibody is specific for Tryosine Hydroxylase, when phosphorylated at serine 31.
Purification Affinity chromatography
Alternative Name Tyrosine 3-monooxygenase (TH)
Background Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines, dopamine (DA), norepinephrine and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C- terminal catalytic domain. Phosphorylation of TH has been shown to occur at several serine residues, including serine 8, 19, 31 and 40. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson.
Alternate names: TYH, Tyrosine 3-hydroxylase
Gene ID 25085
NCBI Accession NP_036872.1
UniProt P04177
Research Area Amino Acids, Enzymes
Application Notes Immunohistochemistry on frozen sections: 1: 1000. Western Blot: 1: 1000, detects a band of approximately 60kDa in rat PC-12 cell lysates,following stimulation by Okadaic acid. Immunofluorescence: 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer 10mM HEPES, pH7.5 containing 0.09% Sodium Azide, 0.01% Bovine Serum Albumin and 50% Glycerol
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Haycock: "Phosphorylation of tyrosine hydroxylase in situ at serine 8, 19, 31, and 40." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 11682-91, 1990 (PubMed).

Lew, Garcia-Espana, Lee et al.: "Increased site-specific phosphorylation of tyrosine hydroxylase accompanies stimulation of enzymatic activity induced by cessation of dopamine neuronal activity." in: Molecular pharmacology, Vol. 55, Issue 2, pp. 202-9, 1999 (PubMed).

Bevilaqua, Graham, Dunkley et al.: "Phosphorylation of Ser(19) alters the conformation of tyrosine hydroxylase to increase the rate of phosphorylation of Ser(40)." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40411-6, 2001 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN319110
819.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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