14-3-3 Pan (pSer58) antibody

Antigen: 14-3-3 Pan

Epitope: pSer58

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Western Blotting (WB)

Catalog no.: ABIN319137

Additional Information

Characteristics: Synonyms: YWHA, Protein kinase C inhibitor protein 1

Alternative name: 14-3-3 protein pan

Immunogen: Synthetic phosphopeptide corresponding to an amino acid sequence within 14-3-3 proteinwhich includes phosphorylated Ser58.

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: The family of 14-3-3 proteins are highly conserved proteins expressed abundantly in thebrain, which have been identified as crucial regulators of many cellular processesincluding multiple signalling pathways, cell growth, apoptosis, intracellular trafficking andmalignant transformation. The interaction of 14-3-3 with target proteins is typicallydependant upon phosphorylation, occurring through phosphoserine andphosphothreonine binding, with 14-3-3 protein activity itself being regulated by kinasessuch as kinase A and C, Akt, and CaMK1 and equally by phosphatases such as proteinphosphatase 1 (PP1) and 2A (PP2A). The ability of 14-3-3 protein to facilitate the phosphorylation of Tau present inneurofibrillary tangles (NFTs) and the co-localisation of 14-3-3 protein with alpha-synucleinfound in Lewy bodies, suggests a role for 14-3-3 protein in the pathogenesis of bothAlzheimer

Specificity: This antibody specifically recognises the 29kDa 14-3-3 protein, when phosphorylated atSer58. Species: Human. Others not tested.

Application Details

Application Notes: Western Blot: 1: 1000, detects a band of approximately 29kDa in rat brainstem cell lystates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: 10mM HEPES, pH7.5 containing 0.09% Sodium Azide, 0.01% Bovine SerumAlbumin, 50% Glycerol

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Caution: (A full Health and Safety assessment is available upon request) This product containsSodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled bytrained staff only.

Restrictions: For Research Use only