This antibody is specific for glutamate receptor subunit 2 (GluR2), a component of the AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid) group of ionotropic glutamate receptors, which play a key role at excitatory synapses, including synaptic transmission, stabilisation and plasticity.
Purification
Affinity chromatography
Immunogen
Keyhole limpet haemocyanin conjugated synthetic peptide corresponding to an amino acid sequence within rat GluR2.
GRIA2
Reactivity: Human, Rat, Mouse
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1: 1000, detects a band of approximately 100 kDa in rat brain hippocampuscell lysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
GluR2 is possibly the most important of the AMPA receptor subunits, responsible for AMPA receptor rectifying properties, control of ion flow and in particular the influx of calcium. The majority of GluR2 in the CNS is expressed in the GluR2(R) form, containing a critical arginine residue (as opposed to a glutamine residue) in the Transmembrane region 2 (TM2) domain, thereby rendering native AMPA receptors impermeable to calcium.Synonyms: AMPA-selective glutamate receptor 2, AMPA2, GRIA2, GluR-B, GluR-K2, Glutamate receptor ionotropic