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Antibodies
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anti-Hepatitis B Surface Antigen (HBSAg) (Subtype ad/ay) antibody
Hepatitis B Surface Antigen (HBSAg) (Subtype ad/ay) antibody
| Antigen |
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Epitope
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| Clonality |
Polyclonal |
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Host
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Application
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Enzyme Immunoassay (EIA) (102), ELISA (23), Immunohistochemistry (Frozen Sections) (IHC (fro)) (17), ELISA (Capture) (16), Immunofluorescence (IF) (15), Western Blotting (WB) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (14), ELISA (Detection) (13), Lateral Flow (LF) (12), Flow Cytometry (FACS) (4), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (IHC) (3), Dot Blot (Dot) (2), Immunoelectrophoresis (IEP) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN319172 |
| Quantity |
1 mg (5.0 mg/ml) |
| Price |
770.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: HBV Large envelope protein, L glycoprotein, Large S protein, Large surface protein, HBVMajor surface antigen
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Alternative name
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Hepatitis B Surface Antigen / HBsAg
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Immunogen
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Hepatitis B Surface Antigen (HBsAg).
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Isotype
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IgG
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Description
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Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage,inflammation and viral persistence. Infection also increases the risk of hepatocellularcarcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists ofpartially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or coreantigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivitybetween the two proteins has been observed. The HBV genome also encodes a DNApolymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but asthe disease progresses this antigen may no longer be present in the blood and tests forHBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronichepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specificcharacteristics of different serotypes and provides the basis of immunodetection. HBsAghas antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y andw/r allow the following combinations: adw, ayw, adr, ayr.
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Specificity
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This antibody is reactive with the Ad and Ay serotypes of the Hepatitis B Virus.
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Application Notes
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ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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5.0 mg/ml
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Purification
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Purified
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Buffer
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PBS containing 0.09% Sodium Azide as preservative
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Alternatives for antigen "Hepatitis B Surface Antigen (HBSAg)", type "Antibodies"
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Hosts
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Mouse (122), Goat (19), Rabbit (14), Horse (1)
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Reactivities
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Hepatitis B (HBV) (56), Human (15)
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Applications
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Enzyme Immunoassay (EIA) (102), ELISA (23), Immunohistochemistry (Frozen Sections) (IHC (fro)) (17), ELISA (Capture) (16), Immunofluorescence (IF) (15), Western Blotting (WB) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (14), ELISA (Detection) (13), Lateral Flow (LF) (12), Flow Cytometry (FACS) (4), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (IHC) (3), Dot Blot (Dot) (2), Immunoelectrophoresis (IEP) (2), Immunoelectron Microscopy (EM) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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HRP (9), Biotin (5), FITC (4)
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Epitopes
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ad/ay (41), AA 132-137 (31), Subtype ad/ay (4), adw/ayw (2), ay (2), ayw (2), Subtype ay (1), Subtype ayw (1), ad (1)
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Alternatives 
