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ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (pSer1177)

Details for Product anti-NOS3 Antibody No. ABIN319232, Supplier: Log in to see
Antigen
Alternatives
anti-Human ENOS antibody for Enzyme Immunoassay
Epitope
pSer1177
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
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Host
Rabbit
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Clonality
Polyclonal
Conjugate
This ENOS antibody is un-conjugated
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-Sp-F-S).
Specificity Phospho-eNOS antibody (AP08011PU) detects endogenous levels of eNOS only when phosphorylated at serine 1177.
Purification Immunoaffinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name NOS3 (NOS3 Antibody Abstract)
Background ENOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial
Gene ID 4846
NCBI Accession NP_000594
UniProt P29474
Research Area Neurotransmitters
Pathways ACE Inhibitor Pathway
Application Notes Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (pSer1177) (ABIN319232) anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody
 image for anti-ENOS antibody (Nitric Oxide Synthase 3 (Endothelial Cell)) (pSer1177) (ABIN319232) anti-Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3) (pSer1177) antibody (Image 2)
Background publications Thomas, Chen, Keaney: "Hydrogen peroxide activates endothelial nitric-oxide synthase through coordinated phosphorylation and dephosphorylation via a phosphoinositide 3-kinase-dependent signaling pathway." in: The Journal of biological chemistry, Vol. 277, Issue 8, pp. 6017-24, 2002 (PubMed).

Harris, Ju, Venema et al.: "Reciprocal phosphorylation and regulation of endothelial nitric-oxide synthase in response to bradykinin stimulation." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16587-91, 2001 (PubMed).

Wadman: "GM advisory panel is slanted, say critics." in: Nature, Vol. 399, Issue 6731, pp. 7, 1999 (PubMed).