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ENOS antibody (Ser1177)

NOS3 Reactivity: Human, Rat, Mouse WB, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN319319
  • Target See all ENOS (NOS3) Antibodies
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Binding Specificity
    • 34
    • 26
    • 22
    • 20
    • 16
    • 16
    • 15
    • 14
    • 12
    • 10
    • 10
    • 8
    • 8
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Ser1177
    Reactivity
    • 265
    • 158
    • 156
    • 14
    • 11
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 275
    • 28
    • 1
    Rabbit
    Clonality
    • 277
    • 27
    Polyclonal
    Conjugate
    • 121
    • 27
    • 16
    • 14
    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 5
    This ENOS antibody is un-conjugated
    Application
    • 255
    • 104
    • 99
    • 91
    • 77
    • 55
    • 37
    • 30
    • 25
    • 24
    • 13
    • 8
    • 7
    • 6
    • 3
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Specificity
    ENOS Antibody AP08055PU detects endogenous levels of total eNOS protein.
    Purification
    Immunoaffinity Chromatography using epitope-specific immunogen.
    Immunogen
    The antiserum was produced against synthesized non-phosphopeptide derived from human eNOS around the phosphorylation site of Serine 1177 (T-Q-SP-F-S).
    Top Product
    Discover our top product NOS3 Primary Antibody
  • Application Notes
    Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store the antibody (in aliquots) at-20 °C.
  • Target
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Alternative Name
    NOS3 (NOS3 Products)
    Synonyms
    ECNOS antibody, eNOS antibody, 2310065A03Rik antibody, Nos-3 antibody, ecNOS antibody, EC-NOS antibody, NOSIII antibody, cNOS antibody, NOS antibody, NOS3 antibody, eNos antibody, ENOS antibody, nitric oxide synthase 3 antibody, nitric oxide synthase 3, endothelial cell antibody, NOS3 antibody, Nos3 antibody, nos3 antibody
    Background
    ENOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.Synonyms: Constitutive NOS, EC-NOS, Endothelial NOS, NOS type III, Nitric oxide synthase, eNOS, endothelial
    Gene ID
    4846
    NCBI Accession
    NP_000594
    UniProt
    P29474
    Pathways
    ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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