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Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody

Details for Product No. ABIN319368, Supplier: Log in to see
Antigen
Epitope
N-Term
36
30
28
24
20
18
14
14
14
12
10
8
8
7
7
7
7
6
6
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
279
82
76
17
11
6
5
2
1
1
1
1
1
Host
Rabbit
242
31
5
4
Clonality
Polyclonal
Conjugate
Un-conjugated
21
17
16
16
13
13
3
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
219
135
112
61
36
27
17
14
14
14
10
6
3
Supplier
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Immunogen The antiserum was produced against a synthesized peptide Corresponding to the amino terminal sequence from Human LC3A protein.
Sequence MPSDRPFKQR RSFA
Specificity This antibody detects endogenous levels of total LC3A/MAP1LC3A protein.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat
Purification Immunoaffinity Chromatography using epitope-specific immunogen
Alternative Name LC3 (MAP1LC3A Antibody Abstract)
Background Microtubule-associated MAPILC3A constitutes nearly half of the mass of all the microtubule associated proteins that copurify with brain microtubules. MAP1LC3A is one of three human orthologs of the rat Map1LC3, (named MAP1LC3A, MAP1LC3B, and MAP1LC3C). The three isoforms of human MAP1LC3 exhibit distinct expression patterns in different human tissues and also differ in their post-translation modifications. MAP1LC3A and MAP1LC3C are produced by the proteolytic cleavage after the conserved C-terminal Gly residue, MAP1LC3B does not undergo C-terminal cleavage and exists in a single modified form.Synonyms: Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3A, MAP1 light chain 3-like protein 1, MAP1A / 1B light chain 3 A, MAP1A/1B light chain 3 A, MAP1A/MAP1B LC3 A, MAP1LC3A, Microtubule-associated protein 1 light chain 3 alpha, Microtubule-associated proteins 1A/1B light chain 3A
Gene ID 84557
NCBI Accession NP_115903
UniProt Q9H492
Application Notes Western blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody (ABIN319368) anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody
 image for anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody (ABIN319368) anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (N-Term) antibody (Image 2)
Background publications He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Hammarback, Obar, Hughes et al.: "MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domain." in: Neuron, Vol. 7, Issue 1, pp. 129-39, 1991 (PubMed).