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Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (N-Term) antibody

Details for Product No. ABIN319372, Supplier: Log in to see
Antigen
Alternatives
anti-Human Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunoprecipitation
Epitope
N-Term
22
17
13
11
9
9
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
104
71
54
11
6
6
5
3
2
1
Host
Rabbit
101
11
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
99
42
24
23
14
12
11
5
2
2
Supplier
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Immunogen The antiserum was produced against a synthesized peptide Corresponding to the amino terminal sequence from human PP2A-alpha protein (''KQLSESQVKSL'').
Specificity PP2A-alpha antibody detects endogenous levels of total PP2A-alpha protein.
Purification Immunoaffinity Chromatography.
Alternative Name PPP2CA (PPP2CA Antibody Abstract)
Background Protein phosphatase 2A (PP2A) is the most abundant phosphatase specific for serine and threonine phosphorylation in mammalian cells. Dephosphorylation of substrates targeted by PP2A plays a vital role in a large variety of cellular processes including signal transduction, regulation of the cell cycle, gene expression and development. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit alpha serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory beta subunit.Synonyms: PP2A-alpha, Protein Phospatase 2A, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Gene ID 5515
NCBI Accession NP_002706
UniProt P67775
Research Area Kinases/Phosphatases, Signaling, Protein Modifications
Pathways PI3K-Akt Signaling
Application Notes Western Blot (1/500-1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (N-Term) antibody (ABIN319372) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (N-Term) antibody
Background publications Janssens, Goris: "Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling." in: The Biochemical journal, Vol. 353, Issue Pt 3, pp. 417-39, 2001 (PubMed).

Yu, Du, Moreno et al.: "Methylation of the protein phosphatase 2A catalytic subunit is essential for association of Balpha regulatory subunit but not SG2NA, striatin, or polyomavirus middle tumor antigen." in: Molecular biology of the cell, Vol. 12, Issue 1, pp. 185-99, 2001 (PubMed).

Tolstykh, Lee, Vafai et al.: "Carboxyl methylation regulates phosphoprotein phosphatase 2A by controlling the association of regulatory B subunits." in: The EMBO journal, Vol. 19, Issue 21, pp. 5682-91, 2000 (PubMed).