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Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody

Details for Product No. ABIN319382, Supplier: Log in to see
Antigen
Epitope
Tyr88
54
27
20
19
15
14
13
13
13
10
6
5
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
276
139
126
8
8
4
4
2
2
1
1
1
Host
Rabbit
239
34
3
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
9
6
6
6
5
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
108
94
43
39
37
20
18
8
4
4
2
2
1
Supplier
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Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Cofilin1/Cofilin2 around the phosphorylation site of Tyrosine 88 (A-T-Yp-E-T).
Specificity This antibody detects endogenous levels of total Cofilin-1/2 protein.
Cross-Reactivity (Details) Also reacts to: Cofilin 2
Purification Immunoaffinity Chromatography.
Alternative Name Cofilin-1 (CFL1 Antibody Abstract)
Background Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan. Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin.Synonyms: 18 kDa phosphoprotein, CFL, CFL1, Cofilin non-muscle isoform, p18
Gene ID 1072
NCBI Accession NP_005498
UniProt P23528
Application Notes Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody (ABIN319382) anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody
 image for anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody (ABIN319382) anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody (Image 2)
 image for anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody (ABIN319382) anti-Cofilin 1 (Non-Muscle) (CFL1) (Tyr88) antibody (Image 3)
Background publications Chen, Cizeau, Vande Velde et al.: "Nix and Nip3 form a subfamily of pro-apoptotic mitochondrial proteins." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 7-10, 1999 (PubMed).

Arber, Barbayannis, Hanser et al.: "Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase." in: Nature, Vol. 393, Issue 6687, pp. 805-9, 1998 (PubMed).