Spleen tyrosine Kinase (SYK) (AA 321-325) antibody

Details for Product No. ABIN319415, Supplier: Log in to see
Antigen
  • p72-Syk
  • Sykb
  • p72syk
  • Tyrosine-protein kinase SYK
  • spleen tyrosine kinase
  • ksyk
  • SYK
  • Syk
Alternatives
anti-Human Spleen tyrosine Kinase antibody for Immunocytochemistry
Epitope
AA 321-325
53
38
31
24
24
16
15
14
13
12
12
10
9
9
9
8
6
5
5
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
376
161
133
13
11
9
8
8
4
3
1
1
Host
Rabbit
276
92
19
Clonality
Polyclonal
Conjugate
Un-conjugated
14
11
10
8
6
6
3
2
2
2
2
2
2
2
2
2
2
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
302
142
122
80
76
48
27
24
14
8
4
4
3
3
2
2
1
1
1
1
1
Options
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Immunogen Peptide sequenece around amino acids 321~325 (N-P-Y-E-P) derived from Human Syk.
Specificity This antibody detects endogenous levels of total Syk protein.
Purification Immunoaffinity Chromatography using epitope-specific immunogen.
Alternative Name SYK (SYK Antibody Abstract)
Background Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK
Gene ID 6850
NCBI Accession NP_001128524
UniProt P43405
Research Area Signaling
Pathways Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling
Application Notes Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (ABIN319415) anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody
 image for anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (ABIN319415) anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (Image 2)
 image for anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (ABIN319415) anti-Spleen tyrosine Kinase (SYK) (AA 321-325) antibody (Image 3)
Background publications Zhang, Billingsley, Kincaid, Siraganian: "Phosphorylation of Syk activation loop tyrosines is essential for Syk function. An in vivo study using a specific anti-Syk activation loop phosphotyrosine antibody." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35442-7, 2000 (PubMed).

Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

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