CD68 antibody

Catalog No. ABIN319454

Product Details anti-CD68 Antibody

Target: CD68 (CD68 Molecule (CD68))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Specificity: CD68 Antibody (Cat#BM4000) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity Chromatography on Protein G

Immunogen: Rat spleen cells. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.

Clone: ED1

Isotype: IgG1

Application Details

Application Notes: RIA. Western blotting. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted CD68 antibody to label 10^6 cells in 100 μL(Results may be enhanced using membrane permeabilisation). Immunohistology on Frozen Sections. Immunohistology on Paraffin Sections (1/100): Requires protein digestion pretreatment ofParaffin sections with Pronase or Trypsin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD68 antibody (ABIN319454)

Meyer, Cunea, Sonntag-Bensch, Welker, Licha, Holz, Schmitz-Valckenberg: "In vivo imaging of a new indocyanine green micelle formulation in an animal model of laser-induced choroidal neovascularization." in: Investigative ophthalmology & visual science, Vol. 55, Issue 10, pp. 6204-12, (2014) (PubMed).

Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68 (CD68 Products)

Background: The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin

Gene ID: 287435

NCBI Accession: NP_001026808

UniProt: Q4FZY1