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Heterogeneous Nuclear Ribonucleoprotein C (C1/C2) (HNRNPC) antibody

Details for Product No. ABIN319473
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Antigen
Synonyms MGC53243, HNRPC, AL022939, D14Wsu171e, Hnrpc, Hnrpc1, Hnrpc2, hnRNPC1, hnRNPC2, hnrnp-C, bZ1G18.4, fb57h12, fi16b11, wu:fb57h12, wu:fi16b11, zgc:55701, C1, C2, HNRNP, SNRPC, hnRNP C, HNRNPC
Reactivity
Chicken, Hamster, Human, Monkey
(47), (34), (33), (14), (12), (3), (3), (3), (3), (2), (1)
Host
Mouse
(44), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(41), (21), (20), (18), (12), (11), (10), (10), (6)
Pubmed 4 references available
Quantity 50 µL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN319473
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Immunogen RNP's eluted from oligo (dT) cellulose column.
Clone 4F4
Isotype IgG1
Specificity This antibody recognizes the two hnRNP C proteins of 41 (C1) and 43kDa (C2) in human cells, and the corresponding proteins in Monkey Hamster and Chicken. This antibody cross react with Mouse in Immunoprecipitation.
Purification Protein A Chromatography.
Alternative Name hnRNP-C1/C2 / HNRNPC
Background The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The HNRNP proteins have distinct nucleic acid binding properties. HNRNP C1 and C2 are encoded by one gene, the two alternatively spliced transcript variants have been described.
Alternate names: HNRPC, Heterogeneous nuclear ribonucleoproteins C1/C2, hnRNP C1 / hnRNP C2
Gene ID 3183
NCBI Accession NP_001070910.1
UniProt P07910
Application Notes ELISA. Western blot (as a guideline try at 1/100000 after antigen concentration). Immunoprecipitation. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Immunofluorescence protocol - Formaldehyde fixationCollect cells from T. c. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Incubate cells in pre-warm (37°C) Para-Formaldehyde for 12 minutes at room temperatureon an orbital shaker. Remove PFA and incubate in 0. 5% Triton X-IOO in 1x PBS for 5 minutes at roomtemperature. Prepare blocking reagent, this is also the antibody diluent. Wash cells 2x with 1x PBS at room temperature, for 4 minutes/wash on an orbital shaker. Block with 1 % NCS and 1x PBS for 30 minutes at room temperature. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. Wash cells 2x with lx PBS at room temperature and air dry briefly. Incubate with primary antibody for 1 hr at room temperature in the dark in stainingchambers. During this time prepare the secondary antibody. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)Incubate with secondary antibody for 1 hour at room temperature in the dark in stainingchambers. Wash cells 5x with 1x PBS. Mount in Dapi. Solutions (prepare fresh the same day of staining): 1x Phosphate buffered saline. Blocking reagent: 1% NCS in 1x PBS (use fresh l0x PBS). Fixation solution: 3. 5% Para formaldehyde. 1. 75g PFA in 20 ml d. H20 plus 5 drops 1M NaOH. Stir on a hot plate at 50-60°C untildissolved. Add 4 drops IN HCI and check pH indicator strip. PH should be 7. 4. Completevolume with d. H20 to 25ml and add 25ml 2xPBS. Check pH before adding to cover slips. Immunofluorescence protocol - Methanol/acetone fixationCollect cells from T. C. unit and remove media from petri dish using suction. Wash with 1x PBS and remove. Fix cells with cold methanol: acetone 1: 1 for 10 minutes on ice. Prepare blocking reagent, this is also the diluent for the antibodies. Remove fixative and wash cells 3x with Ix PBS at RT, for 4 minutes/wash on orbital shaker. Block with 1% NCS and Ix PBS for 30 minutes at RT. Prepare primary antibodies (50μl/coverslip) and moist staining chambers. Wash cells 2x with 1 x PBS at RT and air dry for approximately 7 minutes. Incubate with primary antibody for 1 hr at RT in the dark in staining chambers. During thistime prepare secondary antibody. Wash cells 5x with 1x PBS (5 beaker changes/5 counts in each beaker)Incubate with secondary antibody for 1 hr at R T in the dark in staining chambers. Wash cells 5x with 1x PBS. Mount in Dapi. Solutions (prepare fresh the same day of staining): 1x Phosphate buffered saline. Blocking reagent: 1% NCS in 1x PBS (use fresh 10x PBS).
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS with 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Dreyfuss, Choi, Adam: "Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies." in: Molecular and cellular biology, Vol. 4, Issue 6, pp. 1104-14, 1984 (PubMed).

Choi, Dreyfuss: "Isolation of the heterogeneous nuclear RNA-ribonucleoprotein complex (hnRNP): a unique supramolecular assembly." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 23, pp. 7471-5, 1985 (PubMed).

Zhang, Tai, Wong et al.: "Molecular response of leukemia HL-60 cells to genistein treatment, a proteomics study." in: Leukemia research, Vol. 31, Issue 1, pp. 75-82, 2006 (PubMed).

Tang, Deng, Wang et al.: "Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 11, pp. 1952-67, 2007 (PubMed).

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