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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C) (PE)

Details for Product anti-PTPRC Antibody No. ABIN319591, Supplier: Log in to see
Antigen
  • PTPRC
  • si:dkeyp-47c5.1
  • B220
  • CD45
  • CD45R
  • GP180
  • L-CA
  • LCA
  • LY5
  • T200
  • Cd45
  • Ly-5
  • Lyt-4
  • loc
  • Lca
  • RT7
  • cd45
Reactivity
Cat (Feline), Human, Mouse (Murine)
923
603
185
69
48
41
38
26
21
11
8
5
5
4
3
3
2
1
1
1
1
1
Host
Rat
960
631
201
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is conjugated to PE
267
198
148
100
38
27
22
20
20
19
16
16
14
14
14
14
10
8
7
6
6
6
5
5
5
5
5
5
5
5
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4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
1418
316
308
269
211
194
174
110
65
47
40
29
26
12
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen Mouse pre-B tumour cells (RAW112).
Clone RA3-6B2
Isotype IgG2a
Specificity CD45R
Purification Affinity Chromatography on Protein G.
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45R is a member of the protein tyrosine phosphatase (PTP) family and a major cell surface glycoprotein. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R represents a restricted form of the CD45 family which primarily recognizes only cells of B lineage from proB cell through mature B lymphocytes and, prior to the availability of anti CD19 MAbs, was commonly used as a pan B cell marker. It also reacts with certain activated T cells, as well as non MHC restricted lytically active lymphokine activated killer (LAK) cells. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T and B cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 10090
UniProt P06800
Pathways
Application Notes Flow cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL distilled water.
Buffer Contains 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Dilute only prior to immediate use
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Background publications Rosmalen, Martin, Dobbs et al.: "Subsets of macrophages and dendritic cells in nonobese diabetic mouse pancreatic inflammatory infiltrates: correlation with the development of diabetes." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 80, Issue 1, pp. 23-30, 2000 (PubMed).

Monteith, Chelack, Davis et al.: "Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues." in: Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, Vol. 60, Issue 3, pp. 193-8, 1996 (PubMed).

Whiteland, Nicholls, Shimeld et al.: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995 (PubMed).

Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, 1983 (PubMed).

Holmes, Langdon, Fredrickson et al.: "Analysis of neoplasms induced by Cas-Br-M MuLV tumor extracts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 679-88, 1986 (PubMed).

Spangrude, Heimfeld, Weissman: "Purification and characterization of mouse hematopoietic stem cells." in: Science (New York, N.Y.), Vol. 241, Issue 4861, pp. 58-62, 1988 (PubMed).

Spangrude, Muller-Sieburg, Heimfeld et al.: "Two rare populations of mouse Thy-1lo bone marrow cells repopulate the thymus." in: The Journal of experimental medicine, Vol. 167, Issue 5, pp. 1671-83, 1988 (PubMed).