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KIT antibody (Mast/stem Cell Growth Factor Receptor)

Details for Product anti-KIT Antibody No. ABIN319616, Supplier: Log in to see
Antigen
  • Bs
  • C-Kit
  • c-kit
  • c-KIT
  • CD117
  • Fdc
  • Gsfsco1
  • Gsfsco5
  • Gsfsow3
  • MGF
  • PBT
  • SCFR
  • SCO1
  • SCO5
  • SOW3
  • Ssm
  • Tr-kit
  • W
Alternatives
anti-Mouse (Murine) KIT antibody for Flow Cytometry
Reactivity
Mouse (Murine)
814
388
130
21
19
16
15
14
2
Host
Rat
632
232
206
4
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This KIT antibody is un-conjugated
107
90
84
71
49
41
15
15
11
11
8
8
8
8
8
8
7
6
5
5
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
379
350
349
174
137
109
89
59
46
38
29
13
11
11
8
4
4
3
2
2
2
2
2
2
Supplier
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Immunogen IL-3 dependent mast cells. Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
Clone 2B8
Isotype IgG2b
Specificity This antibody reacts with CD117 also known as Kit or c-kit receptor. Clone 2B8 recognises an epitope within the extracellular region of CD117.
Purification Affinity Chromatography on Protein G.
Alternative Name CD117 / C-Kit (KIT Antibody Abstract)
Background CD117 is the receptor of the steel factor (Stem Cell Factor). C-kit receptor is expressed on 5-10 % of total adult bone marrow cells including multipotent hematopoietic cells.Synonyms: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
Gene ID 16590
NCBI Accession NP_066922
UniProt P05532
Research Area CD Antigens, Surface Receptors of Immune Cells, Growth Factors, Receptors, Proliferation Markers
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Rico-Vargas, Weiskopf, Nishikawa, Osmond: "c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2845-52, 1994

Manova, Bachvarova, Huang, Sanchez, Pronovost, Velazquez, McGuire, Besmer: "c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 12, pp. 4663-76, 1993

Maeda, Yamagata, Nishikawa, Yoshinaga, Kobayashi, Nishi, Nishikawa: "Requirement of c-kit for development of intestinal pacemaker system." in: Development (Cambridge, England), Vol. 116, Issue 2, pp. 369-75, 1993

Fleming, Alpern, Uchida, Ikuta, Weissman: "Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3760-4, 1993

Takagi, Nakahata, Kubo, Shiohara, Koike, Miyajima, Arai, Nishikawa, Zsebo, Komiyama: "Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3446-53, 1992

Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, 1992