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CD3 antibody (FITC)

CD3 Reactivity: Human, Monkey FACS Host: Mouse Monoclonal UCHT1 FITC

Catalog No. ABIN319654

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
189

90

23

15

15

10

8

8

7

5

3

3

2

1
Human, Monkey
Host
215

45

14

13

9
Mouse
Compatible Secondaries
Clonality
285

7
Monoclonal
Conjugate
88

33

29

23

22

11

7

7

6

5

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is conjugated to FITC
Application
267

81

55

44

43

40

28

23

9

9

8

7

4

3

2

2

2

2

2

2

2

1

1
Flow Cytometry (FACS)

Specificity

This antibody recognises a surface protein of peripheral blood T cells of molecular weight 19 kDa. As a pan T cell marker this antibody is present on more than 95 % of circulating human peripheral T cells.

Purification

Affinity Chromatography on Protein G.

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome. Spleen cells from immunised BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line.

Clone

UCHT1

Isotype

IgG1

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anti-CD3 (CD3) antibody
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF Host: Mouse Monoclonal UCHT1 unconjugated

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

anti-CD3 (CD3) antibody (Biotin)
CD3 Reactivity: Human, Non-Human Primate FACS, IP, IHC (fro) Host: Mouse Monoclonal UCHT1 Biotin

anti-CD3 (CD3) antibody (APC)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 APC

anti-CD3 (CD3) antibody (PerCP)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PerCP

anti-CD3 (CD3) antibody (PE)
CD3 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal UCHT1 PE

Application Notes

Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells or 100 μL of lysed whole peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1mg/mL

Buffer

PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing. This antibody is photosensitive and should be protected from light.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

CD3

Alternative Name

CD3 (CD3 Products)

Synonyms

CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody

Background

CD3 is a surface protein of peripheral blood T cells of molecular weight 19 kD. The pan T cell marker is present on more than 95 % of circulating human peripheral T cells.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID

916

NCBI Accession

NP_000724

UniProt

P07766

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway