CD3 (CD3) antibody (PE)

Details for Product No. ABIN319657
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Antigen
Synonyms CD3
Reactivity
Human, Monkey
(485), (137), (59), (28), (24), (16), (12), (7), (6), (5), (5), (3), (2)
Host
Mouse
(514), (88), (73), (46)
Clonality (Clone)
Monoclonal ()
Conjugate
PE
(119), (74), (73), (27), (7), (7), (6), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(524), (107), (106), (101), (94), (92), (66), (48), (47), (28), (23), (10), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 11 references available
Quantity 25 tests
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN319657
198.00 $
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Immunogen Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
Clone UCHT1
Isotype IgG1
Specificity This antibody recognises CD3, a 19 kDa cell surface protein which is expressed on more than 95 % of circulating peripheral blood T-cells.
Purification Affinity chromatography on Protein G
Alternative Name CD3
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with distilled water.
Concentration 0.1 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Kung, Goldstein, Reinherz et al.: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).

Clark, Ledbetter: "Leukocyte cell surface enzymology: CD45 (LCA, T200) is a protein tyrosine phosphatase." in: Immunology today, Vol. 10, Issue 7, pp. 225-8, 1989 (PubMed).

Erber, Mynheer, Mason: "APAAP labelling of blood and bone-marrow samples for phenotyping leukaemia." in: Lancet, Vol. 1, Issue 8484, pp. 761-5, 1986 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Grogan, Fielder, Rangel et al.: "Peripheral T-cell lymphoma: aggressive disease with heterogeneous immunotypes." in: American journal of clinical pathology, Vol. 83, Issue 3, pp. 279-88, 1985 (PubMed).

Campana, Thompson, Amlot et al.: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Clevers, Alarcon, Wileman et al.: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Meuer, Acuto, Hussey et al.: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Clark, Martin, Hansen et al.: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human \T\" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody."" in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

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