Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) antibody (FITC)

Details for Product No. ABIN319724
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Antigen
Synonyms ENTPD1, MGC80631, zgc:100819, entpd1, MGC79567, ATPDase, CD39, NTPDase-1, 2610206B08Rik, AA408691, Cd39
Reactivity
Human
(73), (35), (19), (13), (13)
Host
Mouse
(36), (36), (15), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
(16), (12), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(62), (42), (13), (12), (10), (10), (6), (3), (3), (2), (1), (1)
Pubmed 3 references available
Quantity 25 μg
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Catalog No. ABIN319724
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Immunogen PHA activated human lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone A1
Isotype IgG1
Specificity This antibody binds to the Human CD39 cell surface antigen. This antibody has been shown to block MHC independent target cell recognition by hapten-specific CTL. SM1101A is recommended for this purpose.
Purification Affinity Chromatography on Protein G.
Alternative Name CD39 / ENTPD1
Background CD39 is an 80kD molecule expressed on peripheral blood B cells, monocytes and T cell clones. CD39 is also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.Synonyms: ATPDase, Ecto-ATP diphosphohydrolase, Ecto-apyrase, Ectonucleoside triphosphate diphosphohydrolase 1, Lymphoid cell activation antigen, NTPDase 1
Gene ID 953
NCBI Accession NP_001091645
UniProt P49961
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Suranyi, Bishop, Clayberger et al.: "Lymphocyte adhesion molecules in T cell-mediated lysis of human kidney cells." in: Kidney international, Vol. 39, Issue 2, pp. 312-9, 1991 (PubMed).

Aversa, Suranyi, Waugh et al.: "Detection of a late lymphocyte activation marker by A1, a new monoclonal antibody." in: Transplantation proceedings, Vol. 20, Issue 1, pp. 49-52, 1988 (PubMed).

Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

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