ITGA2 antibody

Catalog No. ABIN319765

Product Details anti-ITGA2 Antibody

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the integrin alpha 2 subunit, which is 160 kDa cell surface antigen expressed by NK cells and a small subset of T-cells. This antibody inhibits cell attachment to collagen.

Purification: Affinity Chromatography on Protein G.

Immunogen: Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.

Clone: AK7

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 1/50-1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Recommended positive control tissue: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: CD49b / ITGA2 (ITGA2 Products)

Synonyms: BR antibody, CD49B antibody, GPIa antibody, HPA-5 antibody, VLA-2 antibody, VLAA2 antibody, DX5 antibody, integrin subunit alpha 2 antibody, integrin alpha 2 antibody, ITGA2 antibody, Itga2 antibody

Background: Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2

Gene ID: 3673

NCBI Accession: NP_002194

UniProt: P17301

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex