CD51/CD61 Complex antibody

Details for Product No. ABIN319776
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Antigen
Reactivity
Chicken, Human
(3), (3)
Host
Mouse
(3)
Clonality (Clone)
Monoclonal ()
Application
Immunoprecipitation (IP), Flow Cytometry (FACS)
(3), (3), (1)
Pubmed 3 references available
Catalog no. ABIN319776
Quantity 25 µg
Price
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Immunogen Osteoclasts from osteoclastomas. Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
Clone 23C6
Isotype IgG1
Specificity This antibody recognises the intact complex formed between the CD51 and CD61 molecules (alpha V and beta 3 integrins). This complex binds vitronectin at the RGD sequence and can also bind fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. This antibody reacts with osteoclasts, placenta, melanoma cell lines and weakly with platelets.
Purification Affinity Chromatography on Protein A.
Background CD51/CD61 is an integrin complex known as alphaV beta3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or beta3 integrin. It is a 110 kD common beta subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components.
Alternate names: CD51, CD61, GP3A, GPIIIa, ITGAV, ITGB-3, ITGB3, Integrin alpha-V, Integrin beta-3, MSK8, Platelet membrane glycoprotein IIIa, VNRA, Vitronectin Receptor, Vitronectin receptor subunit alpha
NCBI Accession 9606
UniProt P06756
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 ul of 1/40-1/80 diluted antibody to label 10e6 cells in 100 ul. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Simpson, Horton: "Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit." in: British journal of experimental pathology, Vol. 70, Issue 3, pp. 257-65, 1989 (PubMed).

Davies, Warwick, Totty et al.: "The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor." in: The Journal of cell biology, Vol. 109, Issue 4 Pt 1, pp. 1817-26, 1989 (PubMed).

Horton, Lewis, McNulty et al.: "Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens." in: Cancer research, Vol. 45, Issue 11 Pt 2, pp. 5663-9, 1985 (PubMed).

Hosts (3)
Reactivities (3), (3)
Applications (3), (3), (1)
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