CD51 antibody (Integrin alpha V)

Details for Product anti-ITGAV Antibody No. ABIN319776, Supplier: Log in to see
Antigen
  • 1110004F14Rik
  • 2610028E01Rik
  • CD51
  • D430040G12Rik
  • MSK8
  • VNRA
  • VTNR
  • Cd51
  • integrin, alpha V
  • integrin alpha V
  • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
  • ITGAV
  • Itgav
Alternatives
anti-Human CD51 antibody for Blocking Peptide
Reactivity
Chicken, Human
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50
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Host
Mouse
256
94
39
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD51 antibody is un-conjugated
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21
20
14
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9
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9
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5
5
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Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
280
94
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41
33
27
14
6
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5
5
4
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2
2
1
1
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Options
Supplier
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Immunogen Osteoclasts from osteoclastomas. Spleen cells from immunised mice were fused with cells of the mouse X63.Ag8.653 myeloma cell line.
Clone 23C6
Isotype IgG1
Purification Affinity Chromatography on Protein A.
Alternative Name CD51 / ITGAV (ITGAV Antibody Abstract)
Background CD51/CD61 is an integrin complex known as alphaV beta3. It is expressed at high levels on osteoclasts, endothelial cells, and melanoma cells and at low levels on platelets and macrophages. CD51 is a heterodimer composed of disulfide-linked 125 kD and 24 kD proteins. CD61 is also a member of the integrin family known as gpIIIa or beta3 integrin. It is a 110 kD common beta subunit of CD51/CD61 or CD41/CD61 complex. CD51/CD61, also known as the vitronectin receptor, mediates the binding of platelets to immobilized vitronectin without prior activation. Other ligands include RGD-containing proteins such as fibrinogen, fibronectin, von Willebrand factor (vWf), laminin, thrombospondin and the neural adhesion molecule L1. CD51/CD61 also mediates cell-cell adhesion via interaction with CD31. CD51/CD61 acts as an activation-independent receptor for platelet attachment and spreading on vitronectin and other RGD-containing proteins, including matrix components.Synonyms: CD51, CD61, GP3A, GPIIIa, ITGAV, ITGB-3, ITGB3, Integrin alpha-V, Integrin beta-3, MSK8, Platelet membrane glycoprotein IIIa, VNRA, Vitronectin Receptor, Vitronectin receptor subunit alpha
Gene ID 9606
UniProt P06756
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex
Application Notes Flow Cytometry: Use 10 μL of 1/40-1/80 diluted antibody to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Simpson, Horton: "Expression of the vitronectin receptor during embryonic development; an immunohistological study of the ontogeny of the osteoclast in the rabbit." in: British journal of experimental pathology, Vol. 70, Issue 3, pp. 257-65, 1989 (PubMed).

Davies, Warwick, Totty, Philp, Helfrich, Horton: "The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor." in: The Journal of cell biology, Vol. 109, Issue 4 Pt 1, pp. 1817-26, 1989 (PubMed).

Horton, Lewis, McNulty, Pringle, Chambers: "Monoclonal antibodies to osteoclastomas (giant cell bone tumors): definition of osteoclast-specific cellular antigens." in: Cancer research, Vol. 45, Issue 11 Pt 2, pp. 5663-9, 1985 (PubMed).