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CD151 antibody (CD151 Molecule (Raph Blood Group))

Details for Product anti-CD151 Antibody No. ABIN319866, Supplier: Log in to see
Antigen
  • CD151
  • gp27
  • GP27
  • MER2
  • mer2
  • MGC83668
  • PETA-3
  • peta-3
  • raph
  • RAPH
  • SFA-1
  • SFA1
  • sfa1
  • TSPAN24
  • tspan24
  • Tspan24
Reactivity
Human
133
31
24
6
2
1
Host
Mouse
94
48
7
Clonality (Clone)
Monoclonal ()
Conjugate
This CD151 antibody is un-conjugated
15
12
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
94
65
52
31
26
18
16
11
4
3
3
3
Supplier
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Immunogen Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Clone 11G5a
Isotype IgG1
Specificity This antibody recognizes the CD151 cell surface antigen, also known as PETA-3.
Purification Affinity Chromatography on Protein G
Alternative Name CD151 / TSPAN24 (CD151 Antibody Abstract)
Background CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen.Synonyms: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24
Gene ID 977
NCBI Accession NP_001034579
UniProt P48509
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes ELISA. Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL of whole blood. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/100. This product does not require protein digestion pre-treatment but results are enhanced with heat mediated antigen retrieval in 0.01M citrate buffer. Recommended positive control: Tonsil/spleen. Western Blot. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions .
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Franco, Muratori, Corso, Tenaglia, Bertotti, Capparuccia, Trusolino, Comoglio, Tamagnone: "The tetraspanin CD151 is required for Met-dependent signaling and tumor cell growth." in: The Journal of biological chemistry, Vol. 285, Issue 50, pp. 38756-64, 2010 (PubMed).

Hasegawa, Furuya, Kasuya, Nishiyama, Sugiura, Nikaido, Momota, Ichinose, Kimura: "CD151 dynamics in carcinoma-stroma interaction: integrin expression, adhesion strength and proteolytic activity." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 87, Issue 9, pp. 882-92, 2007 (PubMed).

Zheng, Liu: "CD151 gene delivery activates PI3K/Akt pathway and promotes neovascularization after myocardial infarction in rats." in: Molecular medicine (Cambridge, Mass.), Vol. 12, Issue 9-10, pp. 214-20, 2007 (PubMed).

Zheng, Liu: "CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation." in: Acta pharmacologica Sinica, Vol. 28, Issue 1, pp. 66-72, 2006 (PubMed).

Karamatic Crew, Burton, Kagan, Green, Levene, Flinter, Brady, Daniels, Anstee: "CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin." in: Blood, Vol. 104, Issue 8, pp. 2217-23, 2004 (PubMed).

Yauch, Berditchevski, Harler, Reichner, Hemler: "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." in: Molecular biology of the cell, Vol. 9, Issue 10, pp. 2751-65, 1998 (PubMed).