CD151 Molecule (Raph Blood Group) (CD151) antibody (PE)

Details for Product No. ABIN319867
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Antigen
Synonyms CD151, MGC83668, gp27, mer2, raph, sfa1, peta-3, tspan24, PETA-3, SFA-1, Tspan24, GP27, MER2, RAPH, SFA1, TSPAN24
Reactivity
Human
(63), (20), (20), (17), (17), (4), (2), (1)
Host
Mouse
(40), (32)
Clonality (Clone)
Monoclonal ()
Conjugate
PE
(8), (6), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(43), (36), (28), (11), (11), (10), (8), (6), (1), (1)
Pubmed 2 references available
Quantity 25 tests
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Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN319867
253.00 $
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Immunogen Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
Clone 11G5a
Isotype IgG1
Specificity This antibody recognises the CD151 cell surface antigen, also known as PETA-3.
Purification Affinity Chromatography on Protein G
Alternative Name CD151 / TSPAN24
Background CD151 is expressed by epithelial cells, endothelial cells, platelets, megakaryocytes, monocytes and in the renal glomeruli and proximal and distal tubules. CD151 is not expressed by lymphocytes or granulocytes. More recently CD151 has also been shown to be expressed by erythrocytes, and to carry the MER2 blood group antigen. (2) It should be noted that CD151 is very closely associated with the alpha3 beta1 integrin in cells and coimmunoprecipitation may occur even in quite stringent conditions. (1)
Alternate names: GP27, Membrane glycoprotein SFA-1, PETA-3, PETA3, Platelet-endothelial tetraspan antigen 3, SFA-1, SFA1, Tetraspanin-24
Gene ID 977
NCBI Accession NP_001034579
UniProt P48509
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Use 10 ul of neat antibody to label 10e6 cells in 100 ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL distilled water
Buffer contains 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Yauch, Berditchevski, Harler et al.: "Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration." in: Molecular biology of the cell, Vol. 9, Issue 10, pp. 2751-65, 1998 (PubMed).

Karamatic Crew, Burton, Kagan et al.: "CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin." in: Blood, Vol. 104, Issue 8, pp. 2217-23, 2004 (PubMed).

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