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Details for Product No. ABIN319885

B-Cell CLL/lymphoma 2 (BCL2) (AA 41-54) antibody

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Antigen
Synonyms Bcl-2, PPP1R50, bcl-2, BCL-2
Epitope
»Alternatives AA 41-54
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 4 references available
Catalog no. ABIN319885
Quantity 20 µg
Price
220.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide containing aa 41-54 of the bcl-2 protein.
Clone LL002
Isotype IgG1
Specificity This antibody reacts specifically with the 25kD Bcl-2 oncoprotein. This antibody reacts with small B lymphocytes in the mantle zone and many cells within T cell areas. Very few cells in germinal centres are stained. In the thymus many cells in the medulla are stained but the cortex shows weak or negative staining. In non-haematopoietic tissues few cells are stained. The antibody reacts with neoplastic cells of follicular lymphoma and anaplastic large cell lymphoma.
Purification Affinity chromatography on Protein G
Alternative Name Bcl-2
Background Bcl-2 is an integral membrane protein which lies within the cell rather than on the cell surface. The protein is localised in mitochondria and plays a role in the inhibition of apoptosis.
Alternate names: Apoptosis regulator Bcl-2, B-Cell CLL/lymphoma 2, BCL2, Bcl-2 alpha
Gene ID 596
NCBI Accession NP_000624.2
UniProt P10415
Research Area Cancer, Apoptosis/Necrosis
Application Notes Immunoprecipitation. Western Blot. Flow Cytometry (1/50-1/100): Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections: (1/20-1/40). Immunohistochemistry on Paraffin Sections: (1/20-1/40): This product requires antigen retrieval using heat treatment prior to staining of formalin fixed material Fixation using BS or Bouins fluid is preferable to formalin fixation. Recommended Positive Control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Pezzella, Tse, Cordell et al.: "Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation." in: The American journal of pathology, Vol. 137, Issue 2, pp. 225-32, 1990 (PubMed).

Hockenbery, Nuñez, Milliman et al.: "Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death." in: Nature, Vol. 348, Issue 6299, pp. 334-6, 1991 (PubMed).

Cleary, Smith, Sklar: "Cloning and structural analysis of cDNAs for bcl-2 and a hybrid bcl-2/immunoglobulin transcript resulting from the t(14;18) translocation." in: Cell, Vol. 47, Issue 1, pp. 19-28, 1986 (PubMed).

Tsujimoto, Croce: "Analysis of the structure, transcripts, and protein products of bcl-2, the gene involved in human follicular lymphoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5214-8, 1986 (PubMed).

Alternatives for antigen "B-Cell CLL/lymphoma 2 (BCL2)", type "Antibodies"
Hosts (311), (128), (9), (5), (1)
Reactivities (400), (149), (118), (61), (38), (36), (14), (12), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Applications (302), (150), (141), (102), (95), (75), (70), (50), (50), (47), (12), (11), (4), (3), (2), (2), (2), (2), (1), (1)
Conjugates (15), (14), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (1), (1), (1), (1)
Epitopes (43), (37), (30), (25), (18), (17), (16), (13), (8), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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