Synaptosomal-Associated Protein, 25kDa (SNAP25) antibody

Details for Product No. ABIN319890
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Synonyms ric-4, ric4, sec9, snap-25, SNAP-25B, SNAP-25a, Bdr, SNAP-25, sp, RIC-4, RIC4, SEC9, SNAP, bA416N4.2, dJ1068F16.2, fj55h11, snap25, wu:fj55h11
Hamster, Human, Pig (Porcine), Rat (Rattus)
(272), (118), (107), (29), (20), (14), (14), (14), (11), (7), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1)
(146), (139), (23), (7), (1)
Clonality (Clone)
Monoclonal ()
(10), (9), (7), (7), (7), (7), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(246), (151), (101), (65), (47), (45), (27), (17), (17), (11), (10), (8), (6), (3), (3), (2), (2), (1)
Pubmed 10 references available
Quantity 20 μg
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Immunogen Crude Human synaptic immunoprecipitate.
Clone SP12
Isotype IgG1
Specificity This antibody appears to recognize the pre-synaptic protein SNAP-25, an antigen of 26-27 kDa Molecular Weight. It will recognise SNAP-25 fusion protein from COS cells, but not the fusion protein from bacterial systems. Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy. (1,3,8)
Purification Affinity Chromatography on Protein A.
Alternative Name SNAP25 (SNAP25 Antibody Abstract)
Background SNAP25 is a soluble protein of 25 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. This protein was originally discovered to be enriched in subsets of neurons in the brain and displays a presynaptic pattern of expression. The membrane proteins SNAP25, synaptobrevin, and syntaxin form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. Functional interactions have been demonstrated to exist between SNAP-25, syntaxin, and the synaptic protein interaction site on voltage-sensitive L- and N-type calcium channels. SNAP-25 has been shown to be required for synaptic vesicle fusion with the plasma membrane during release of nerve growth factor (NGF). Regulated exocytosis of cortical granule secretion in fertilized eggs and membrane fusion events in neurons and endocrine cells is mediated by SNAP-25 in a calcium-dependent mechanism. SNAP25 protein levels have been shown to be elevated in prolactinoma and growth hormone (GH)/prolactin (PRL) tumor cells while reduced SNAP-25 protein expression has been observed between schizophrenic and normal hippocampal cells. Altered patterns of SNAP-25 expression have also been observed in the inferior temporal cortex and prefrontal association cortex between normal brains and brains from individuals affected with schizophrenia. SNAP25 accumulation due to cytoskeletal dysfunction is also observed in the swollen axons of the white matter of individuals with severe Alzheimer's dementia.Synonyms: RIC4, SNAP, SNAP-25, SUP, Super Protein, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein 25, ric-4
Gene ID 6616
NCBI Accession NP_003072
UniProt P60880
Research Area Cancer, Neurology
Application Notes Western Blot: 1/5000-1/10000. ELISA. Immunohistochemistry on Paraffin Sections: 1/2000-1/5000, This product does not require protein digestion pre-treatment of paraffin sections, This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control tissue: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Simpson, Chundu, Davies-Hill et al.: "Decreased concentrations of GLUT1 and GLUT3 glucose transporters in the brains of patients with Alzheimer's disease." in: Annals of neurology, Vol. 35, Issue 5, pp. 546-51, 1994 (PubMed).

Honer, Beach, Hu et al.: "Hippocampal synaptic pathology in patients with temporal lobe epilepsy." in: Acta neuropathologica, Vol. 87, Issue 2, pp. 202-10, 1994 (PubMed).

Masliah, Honer, Mallory et al.: "Topographical distribution of synaptic-associated proteins in the neuritic plaques of Alzheimer's disease hippocampus." in: Acta neuropathologica, Vol. 87, Issue 2, pp. 135-42, 1994 (PubMed).

Wakabayashi, Honer, Masliah: "Synapse alterations in the hippocampal-entorhinal formation in Alzheimer's disease with and without Lewy body disease." in: Brain research, Vol. 667, Issue 1, pp. 24-32, 1995 (PubMed).

Dickson, Davies, Bevona et al.: "Hippocampal sclerosis: a common pathological feature of dementia in very old (> or = 80 years of age) humans." in: Acta neuropathologica, Vol. 88, Issue 3, pp. 212-21, 1995 (PubMed).

Honer, Hu, Davies: "Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex." in: Brain research, Vol. 609, Issue 1-2, pp. 9-20, 1993 (PubMed).

Dickson, Crystal, Bevona et al.: "Correlations of synaptic and pathological markers with cognition of the elderly." in: Neurobiology of aging, Vol. 16, Issue 3, pp. 285-98; discussion 298-304, 1995 (PubMed).

Honer, Kaufmann, Kleinman et al.: "Monoclonal antibodies to study the brain in schizophrenia." in: Brain research, Vol. 500, Issue 1-2, pp. 379-83, 1990 (PubMed).

Honer, Kaufmann, Davies: "Characterization of a synaptic antigen of interest in neuropsychiatric illness." in: Biological psychiatry, Vol. 31, Issue 2, pp. 147-58, 1992 (PubMed).

Honer, Dickson, Gleeson et al.: "Regional synaptic pathology in Alzheimer's disease." in: Neurobiology of aging, Vol. 13, Issue 3, pp. 375-82, 1992 (PubMed).

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Catalog No. ABIN319890
$ 198.00
Plus shipping costs $45.00
20 μg
$ 198.00

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