CD103 antibody

Catalog No. ABIN320002

Product Details anti-CD103 Antibody

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD103 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody recognises the CD103 cell surface antigen, also known as the alphaE integrin. This reacts with various subsets of intra-epithelial lymphocytes:- 40 % of lamina propria T-cells, 30 % of mesenteric lymphoblasts and some lymphocytes from other mucosa. It recognises less than 0.1 % bone marrow cells and less than 0.7 % PBL.

Purification: Affinity Chromatography on Protein A.

Immunogen: Human Intestinal lymphocyte. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Clone: 2G5-1

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 5 x 10e5 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on Frozen Sections (1/50-1/100). Recommended positive control tissue: intestine.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: CD103 (ITGAE Products)

Synonyms: A530055J10 antibody, CD103 antibody, aM290 antibody, alpha-E1 antibody, alpha-M290 antibody, HUMINAE antibody, integrin alpha E, epithelial-associated antibody, integrin subunit alpha E antibody, Itgae antibody, ITGAE antibody

Background: CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20 % of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150 kDa alpha chain and a 120 kDa beta chain. Integrin alpha E is expressed by approximately 1 % of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.Synonyms: HML-1 antigen, ITGAE, Integrin alpha-E, Integrin alpha-IEL, Mucosal lymphocyte 1 antigen

Gene ID: 3682

NCBI Accession: NP_002199

UniProt: P38570