This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-Myc 'Tag'.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.
MYC
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
7-00E-10
unconjugated
Application Notes
ELISA: 1/500-1/5000. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag