KRT9 antibody (AA 315-456)

Catalog No. ABIN3201430

Product Details anti-KRT9 Antibody

Target: KRT9 (Keratin 9 (KRT9))

Binding Specificity: AA 315-456

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to Cytokeratin 9 (CK9)

Specificity: The antibody is a mouse monoclonal antibody raised against CK9. It has been selected for its ability to recognize CK9 in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Cytokeratin 9 (CK9) corresdonding to Pro315~Asn456 with N-terminal His Tag

Clone: C7

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:360-1460 Immunohistochemistry: 5-20 μg/mL

1:36-146 Immunocytochemistry: 5-20 μg/mL

1:36-146 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for KRT9

Target: KRT9 (Keratin 9 (KRT9))

Alternative Name: Cytokeratin 9 (KRT9 Products)

Synonyms: CK-9 antibody, EPPK antibody, K9 antibody, Krt1-9 antibody, keratin 9 antibody, KRT9 antibody, Krt9 antibody

Background: KRT9, EPPK, K9, Keratin 9, Type I Cytoskeletal 9, Epidermolytic Palmoplantar Keratoderma